05-443
Anti-VEGF Antibody, clone JH
clone JH, Upstate®, from mouse
Synonym(s):
Vascular permeability factor, vascular endothelial growth factor, vascular endothelial growth factor A, vascular endothelial growth factor isoform VEGF165
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
100
400
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
JH, monoclonal
species reactivity
rat, mouse, rabbit, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable (paraffin)
isotype
IgG1κ
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... VEGFA(7422)
General description
The Flt gene family of receptor tyrosine kinases includes Flt-1, KDR/Flk-1, Flt-3, and Flt-4. They encode receptors for VEGF, characterized by seven Ig-loops within their extracellular domain and a split kinase domain within the cytoplasmic domain. VEGF is an important angiogenic factor of many solid tumors and is also involved in the differentiation of endothelial cells. Flt-1 (Fms-like tyrosine kinase, VEGF-R1) is expressed on endothelial cells and on the common precursors of endothelial and hematopoietic stem cells. KDR (Flk-1, VEGF-R2) is the principal receptor involved in VEGF signaling. KDR is expressed on endothelial cells and stimulates angiogenesis, but is also expressed on bone marrow cells, and is thought to play a role in driving the proliferation of most or all leukemic cells. Flt3 is a class III receptor tyrosine kinase that is closely related to c-Kit and c-Fms. It is expressed in primitive hematopoietic cells and its ligand exerts proliferative effects on these cells in co-operation with other cytokines. Flt3 is also expressed the placenta, testis, spleen, thymus, bone marrow, brain and in cerebellar Purkinje cells. The VEGF receptors stimulate the Ras-MAP kinase pathway, suggesting that it signals as a conventional receptor tyrosine kinase.
Specificity
Other species not tested.
Recognizes 34-54 kDa isoforms of Vascular Endothelial Growth Factor/Vascular Permeability Factor (VEGF/VPF).
Immunogen
Recombinant human VEGF121. Clone JH121
Application
Immunohistochemistry: This lot of antibody detected VEGF in formalin fixed, paraffin embedded, human.tissues, using a 1:50 dilution incubated overnight at 4°C.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Immunoprecipitation: 2 µg of this antibody has been reported to immunoprecipitate VEGF from 1 mg of protein lysate.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Anti-VEGF Antibody, clone JH is validated for use in IH(P) for the detection of VEGF.
Quality
Biological Blockade: Incubation of HUVEC cells with 5 µg/mL of a previous lot and 5 ng/mL of VEGF (Catalog # 01-185) has shown growth inhibition of 20-70%.
Target description
34-54 kDa isoforms
Physical form
Format: Purified
Protein G Chromatography
Purified mouse monoclonal IgG1k in buffer containing 10 mM PBS, pH 7.4.
Storage and Stability
Stable for 1 year at from date of receipt.
Analysis Note
Control
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Expressed in most tissue, including Pancreas, Kidney, Adenocarcinoma.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
C C Tassou et al.
Journal of food protection, 67(1), 193-198 (2004-01-14)
The effect of prestorage treatments, such as immersion in a sorbate solution (5%, wt/vol), heating (60 degrees C, 1 min), and a combination of the two treatments, and the subsequent storage in air or under modified atmosphere packaging (MAP; 40%
Hypoxic induction of human vascular endothelial growth factor expression through c-Src activation.
Mukhopadhyay, D, et al.
Nature, 375, 577-581 (1995)
Inducement of mitogen-activated protein kinases in frozen shoulders.
Kanbe, K; Inoue, K; Inoue, Y; Chen, Q
Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association null
Ulrich Sure et al.
Neurosurgery, 55(3), 663-669 (2004-09-01)
In previous studies, we documented a marked neoangiogenesis and endothelial proliferation in cerebral arteriovenous malformations (AVMs) that were embolized before surgery compared with those that were not embolized. We hypothesized that embolization caused a local hypoxia that promotes neoangiogenesis as
Exogenous Smad3 accelerates wound healing in a rabbit dermal ulcer model.
Sumiyoshi, K; Nakao, A; Setoguchi, Y; Okumura, K; Ogawa, H
The Journal of Investigative Dermatology null
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service