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Key Documents

P1484

Sigma-Aldrich

Anti-p21WAF1/Cip1antibody, Mouse monoclonal

clone CP74, purified from hybridoma cell culture

Synonym(s):

Anti-CDKN1A, Anti-Cip1, Anti-WAF1, Anti-p21

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CP74, monoclonal

form

buffered aqueous solution

mol wt

antigen 21 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using LNCap cells (human prostate adenocarcinoma) treated with MG132 proteosome inhibition.

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDKN1A(1026)
mouse ... Cdkn1a(12575)

General description

Monoclonal Anti-p21WAF1/Cip1 (mouse IgG2b isotype) is derived from the CP74 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant human p21WAF1/Cip1. p21WAF1/Cip1 is 21 kDa and designated as Cip1, WAF1, Sdi1, Pic1, CAP20.
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody reacts specifically with p21WAF1/Cip1. Two families of CKIs have been identified. The p21WAF1/Cip1 family contains p21WAF1/Cip1, p27Kip1 and p57Kip2.

Immunogen

recombinant human p21WAF1/Cip1.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Mouse monoclonal clone CP74 anti-p21WAF1/Cip1 antibody may be used for ELISA, immunoblotting (21 kDa) and immunoprecipitation (p21/cyclin A/Cdk2 complexes, native and denatured). The availability of a monoclonal antibody reacting specifically with p21WAF1/Cip1 enables the subcellular detection and localization of p21WAF1/Cip1 and the measurement of relative differences in p21WAF1/Cip1 levels as a function of cell cycle phase.

Biochem/physiol Actions

Regulation of cell cycle progression in eukaryotic cells depends on the expression of proteins called cyclins. These proteins form complexes with several different cyclin dependent kinases (CDKs). The catalytic activity of cyclin dependent kinases (CDK) is regulated by two general mechanisms: protein phosphorylation and association with regulatory subunits, which include the cyclins and the CDK inhibitors (CKIs). These inhibit all kinases involved in the G1/S transition. The the p16INK4a family, which includes p15INK4b, p16INK4a, p18INK4c and p19INK4d, inhibits Cdk4 and Cdk6 specifically.
The p21WAF1/Cip1 inhibit all kinases involved in the G1/S transition of cell cycle. p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is relatively a poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

Physical form

Solution in phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tumor susceptibility of p21Waf1/Cip1-deficient mice
Martin- Caballero J, et al.
Cancer research, 61(16), 6234-6238 (2001)
Yuji Wang et al.
The Journal of biological chemistry, 287(31), 25941-25953 (2012-05-19)
Tumor suppressor genes are frequently silenced in cancer cells by enzymes catalyzing epigenetic histone modifications. The peptidylarginine deiminase family member PAD4 (also called PADI4) is markedly overexpressed in a majority of human cancers, suggesting that PAD4 is a putative target
Purification and analysis of CIP/KIP proteins.
B D Dynlacht et al.
Methods in enzymology, 283, 230-244 (1997-01-01)
T Feng et al.
Oncogenesis, 6(1), e294-e294 (2017-01-31)
TGF-β signaling in liver cells has variant roles in the dynamics of liver diseases, including hepatocellular carcinoma (HCC). We previously found a correlation of high levels of the important endogenous negative TGF-β signaling regulator SMAD7 with better clinical outcome in
Yang Yang et al.
Cell death & disease, 9(9), 886-886 (2018-09-01)
The tumor suppressor p53 plays a pivotal role in the protection against cancer. Increasing evidence suggests that long noncoding RNA (lncRNA) plays an important role in the regulation of the p53 pathway, however, the detailed mechanisms remain to be further

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