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P0090

Sigma-Aldrich

Anti-PMP70−Atto 488 antibody produced in rabbit

1.5-3 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-70 kDa Peroxisonal membrane protein, Anti-ABCD3, Anti-PXMP1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

Atto 488 conjugate

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

rat, mouse, human

packaging

antibody small pack of 25 μL

storage condition

protect from light

concentration

1.5-3 mg/mL

technique(s)

direct immunofluorescence: 10-20 μg/mL using human HeLa cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ABCD3(5825)
mouse ... Abcd3(19299)
rat ... Abcd3(25270)

General description

70 kDa peroxisomal membrane protein (PMP70) belongs to the adrenoleukodystrophy (ALD) subfamily of the ATP-binding cassette (ABC) transporter superfamily. It is a half-size ABC integral membrane protein consisting of 6 transmembrane domains and one ATP-binding domain.
ATP-binding cassette sub-family D member 3 (ABCD3) is a peroxisomal membrane protein belonging to an ATP binding cassette family. The gene encoding it is localized on human chromosome 1p21-22 and rat chromosome 2q41.

Immunogen

synthetic peptide corresponding to amino acid residues 644-659 of rat PMP70 conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in human.

Application

Atto 488 antibody produced in rabbit has been used in immunofluorescence.

Biochem/physiol Actions

70 kDa peroxisomal membrane protein (PMP70) forms a stable complex with the adrenoleukodystrophy protein, (ALDP), and several other peroxisomal proteins. ATP-binding/hydrolysis by PMP70 and ALDL and their phosphorylation are involved in the regulation of fatty acid transport into peroxisomes. Mutations in the PMP70 (PXMP1) gene may cause a subset of Zellweger syndrome-2, an autosomal recessive disorder that is manifested by a defective import mechanisms for peroxisomal matrix enzymes.
ATP-binding cassette sub-family D member 3 (ABCD3) is responsible for the transport of fatty acids into peroxisomes by an ATP-dependent mechanism. It also participates in the oxidation of dicarboxylic acids. Defects in the protein activity result in hepatosplenomegaly, a liver disease. Change in the expression of ABCD3 is associated with prostate tumor aggressiveness and a deficiency of the protein causes bile acid biosynthesis defect.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunohistological analysis of ABCD3 expression in Caucasian and African American prostate tumors.
Reams RR
BioMed Research International (2015)
Immunohistological analysis of ABCD3 expression in Caucasian and African American prostate tumors.
Ferdinandusse S
Human Molecular Genetics (2015)
ABC Transporter Subfamily D: Distinct Differences in Behavior between ABCD1-3 and ABCD4 in Subcellular Localization, Function, and Human Disease.
Kawaguchi K and Morita M
BioMed Research International (2016)
A role for the human peroxisomal half-transporter ABCD3 in the oxidation of dicarboxylic acids.
van Roermund CW
Biochimica et Biophysica Acta (2014)
Kai Wen Teng et al.
eLife, 5 (2016-12-10)
Site-specific fluorescent labeling of proteins inside live mammalian cells has been achieved by employing Streptolysin O, a bacterial enzyme which forms temporary pores in the membrane and allows delivery of virtually any fluorescent probes, ranging from labeled IgG's to small

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