H4149
Monoclonal Anti-Heat Shock Protein 60 antibody produced in mouse
clone LK1, ascites fluid
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About This Item
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biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
LK1, monoclonal
mol wt
antigen 60 kDa
contains
15 mM sodium azide
species reactivity
rat, human, chicken
technique(s)
electron microscopy: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
indirect ELISA: suitable
western blot: 1:400 using cultured human foreskin fibroblast extract
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... HSPD1(3329)
rat ... Hspd1(63868)
General description
Heat shock (stress) proteins (HSP) are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. HSP-60 is a member of HSP family with a uniquely high level of sequence conservation.
Monoclonal Anti-Heat Shock Protein 60 (mouse IgG1 isotype) is derived from the LK1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human heat diseases.
Specificity
The antibody recognizes an epitope located between amino acid residues 383-447 of the human HSP 60. It is not cross-reactive with the bacterial counterpart (E. coli), or with helminth and spinach HSP60. The antibody reacts with constitutive and inducible HSP60. It demonstrates a raised level of staining in immunohistochemistry of synovial membranes taken from patients with juvenile chronic arthritis.
Immunogen
recombinant human heat shock protein 60 (HSP60)
Application
Monoclonal Anti-Heat Shock Protein 60 has been used in the following techniques:
- Enzyme linked immunosorbent assay (ELISA)
- Immunoblotting
- Immunocytochemistry
- Immunohistochemistry
- Immunoelectron microscopy
- Double immunofluorescence
Biochem/physiol Actions
A wide variety of environmental perturbations, such as a sudden increase in temperature, induce cells to rapidly synthesize a group of polypeptides known as the heat shock (stress) proteins. HSP-60 is considered to be a potential antigen in a number of autoimmune diseases. Abnormal immune reactivity involving shock protein 60 (HSP60) has also been implicated in the pathogenesis of schizophrenia. This monoclonal antibody enables the differentiation between the HSP60 of mammalian and bacterial origin.
Abnormal immune reactivity involving HSP60 has also been implicated in the pathogenesis of schizophrenia.
Physical form
Monoclonal Anti-Heat Shock Protein 60 is provided as ascites fluid with 0.1% sodium azide as a preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Antonella Marino Gammazza et al.
Cell stress & chaperones, 19(3), 343-353 (2013-09-24)
The role Hsp60 might play in various inflammatory and autoimmune diseases is under investigation, but little information exists pertaining to Hashimoto's thyroiditis (HT). With the aim to fill this gap, in the present work, we directed our attention to Hsp60
P Costantini et al.
Cell death and differentiation, 9(1), 82-88 (2002-01-23)
As shown here, mitochondria purified from different organs (liver, brain, kidney, spleen and heart) contain both pro-caspase-9 and the processed, mature form of caspase-9. Purified liver mitochondria release mature caspase-9 upon induction of permeability transition in vitro. This is accompanied
Francesco Cappello et al.
Cancer, 107(10), 2417-2424 (2006-10-19)
The relation between smoking, chronic obstructive pulmonary disease (COPD), and lung cancer (LC) is an open field of investigation. A higher frequency of adenocarcinoma has been reported in patients with COPD. Heat shock proteins (Hsps) are implicated in tumoral cell
Claudia Campanella et al.
Oncotarget, 7(20), 28849-28867 (2015-12-25)
HSP60 undergoes changes in quantity and distribution in some types of tumors suggesting a participation of the chaperonin in the mechanism of transformation and cancer progression. Suberoylanilide hydroxamic acid (SAHA), a member of a family of histone deacetylase inhibitors (HDACi)
Studies characterizing 60 kda autoantibodies in subjects with schizophrenia
Wang X, et al.
Biological Psychiatry, 53(5), 361-375 (2003)
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