F8264
Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
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About This Item
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biological source
goat
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:64
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (γ-chain specific)-FITC antibody is specific for is specific for mouse IgG when tested against purified mouse IgA, IgG (all subclasses), and mouse IgM. Goat anti-mouse IgG is isolated by affinity isolation and conjugated to Fluorescein Isothiocyanate (FITC), isomer I.
Immunogen
Purified mouse IgG
Application
Anti-Mouse IgG (γ-chain specific)-FITC antibody may be used for immunofluorescence of mouse spleen cells at a working antibody dilution of 1:64. For ELISA using mice sera, antibody dilution of 1:50 was used. The antibody was also used in anti-nucear antibody detection using mouse sera and for labeling Saimiri brain endothelial cells for exhaustive photon reassignment microscopy
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunofluorescence (1 paper)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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C M Shih et al.
Journal of clinical microbiology, 34(9), 2297-2299 (1996-09-01)
We determined whether the span of infectivity of Lyme disease spirochetes (Borrelia burgdorferi) to vector ticks varies with the mode of infection in laboratory mice. Noninfected larval deer ticks were permitted to feed on two strains of spirochete-infected mice that
C M Shih et al.
Journal of clinical microbiology, 33(12), 3164-3168 (1995-12-01)
We determined whether the infectivity of the Lyme disease spirochete (Borrelia burgdorferi) to vector ticks varies with the duration of infection in laboratory mice. Thus, noninfected nymphal deer ticks were permitted to feed on two strains of early (2 months
Anna A Ogienko et al.
International journal of molecular sciences, 25(2) (2024-01-27)
The nucleolus is a significant nuclear organelle that is primarily known for its role in ribosome biogenesis. However, emerging evidence suggests that the nucleolus may have additional functions. Particularly, it is involved in the organization of the three-dimensional structure of
B Pouvelle et al.
Molecular medicine (Cambridge, Mass.), 3(8), 508-518 (1997-08-01)
Chondroitin-4-sulfate (CSA) was recently described as a Plasmodium falciparum cytoadherence receptor present on Saimiri brain microvascular and human lung endothelial cells. To specifically study chondroitin-4-sulfate-mediated cytoadherence, a parasite population was selected through panning of the Palo-Alto (FUP) 1 P. falciparum
V P Chekhonin et al.
Drug delivery, 12(1), 1-6 (2005-04-02)
Polyethylene glycol (PEG)ylated (stealth) immunoliposomes directed against human gliofibrillary acidic protein (GFAP) were prepared by coupling the thiolated monoclonal anti-GFAP antibodies with a maleimide derivative of phosphatidyl ethanolamine of the liposomal membrane. Experiments with cell cultures demonstrated specific and competitive
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