70722

E. coli ChromoSelect Agar B

NutriSelect^® Plus, suitable for microbiology

• Synonym(s): E. coli Agar B ChromoSelect
• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; bile salt mixture, 1.5 g/L ; casein enzymic hydrolysate, 20 g/L ; X-glucuronide, 0.075 g/L
• manufacturer/tradename: NutriSelect^® Plus
• final pH: 7.2±0.2 (25 °C)
• application(s): agriculture; bioburden testing; environmental; food and beverages; water monitoring; microbiology
• suitability: selective and differential for Escherichia coli; selective and differential for Klebsiella spp.; selective and differential for coliforms

Description

Application

E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Preparation Note

Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable