G005795
HPLC Analysis of Insulin (Species Variants) on Ascentis® Express Phenyl-Hexyl
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express Phenyl-Hexyl, 15 cm x 2.1 mm, 2.7 μm particles (53338-U)
mobile phase: [A] 70:30, water with 0.1% TFA:acetonitrile with 0.09% TFA; [B] 55:45, water with 0.1% TFA:acetonitrile with 0.09% TFA
gradient: 0 to 100% B in 7.5 min
flow rate: 0.3 mL/min
pressure: 3960 psi (273 bar)
column temp.: 30 °C
detector: UV, 215 nm
injection: 0.5 μL
sample: 200 μg/mL in 0.1% formic acid
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Analysis Note
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