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Supelco

SUPELCOSIL™ SAX1 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501

Product Name

SUPELCOSIL™ SAX1 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

Agency

suitable for USP L14

manufacturer/tradename

SUPELCOSIL™

extent of labeling

12% carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

matrix

silica gel, spherical particle platform

matrix active group

propyltrimethylammonium phase

particle size

5 μm

pore size

120 Å

application(s)

food and beverages

separation technique

anion exchange

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General description

SUPELCOSIL™ SAX1 HPLC Column is typically employed as an anion exchange column in chromatographic separations.
The SUPELCOSIL SAX1 column has a strongly basic quaternary aminopropyl phase and is used for separating anions.

Application

SUPELCOSIL™ SAX1 HPLC Column has been used for the separation of:
  • Organoarsenic compounds in marine algae and in biological materials during their analysis using size-exclusion-anion-exchange (SE-AE) high performance liquid chromatography (HPLC) combined with inductively coupled plasma mass spectrometry (ICP-MS).
  • Organoarsenic compounds in edible algae during their analysis using bi-dimensional SE-AE-HPLC combined with dual ICP-MS and electrospray tandem mass spectrometry (ESI-MS/MS).
  • Polypeptides in biological matrices during their analysis using supercritical fluid chromatography (SFC) coupled to MS detector.

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

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Certificates of Analysis (COA)

Lot/Batch Number
280774-04
280774-03
280774-02
280774-01
280647-04

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Gas and liquid chromatography with inductively coupled plasma mass spectrometry detection for environmental speciation analysis-advances and limitations.
Szpunar J, et al.
Spectrochimica Acta. Part B: Atomic Spectroscopy, 55(7), 779-793 (2000)
Speciation of arsenic in edible algae by bi-dimensional size-exclusion anion exchange HPLC with dual ICP-MS and electrospray MS/MS detection.
McSheehy S and Szpunar J
Journal of Analytical Atomic Spectrometry, 15(1), 79-87 (2000)
Feasibility of supercritical fluid chromatography/mass spectrometry of polypeptides with up to 40-mers.
Zheng J, et al.
Analytical Chemistry, 78(5), 1535-1545 (2006)
Trace element speciation analysis of biomaterials by high-performance liquid chromatography with inductively coupled plasma mass spectrometric detection.
Szpunar J.
TrAC, Trends in Analytical Chemistry, 19(2-3), 127-137 (2000)

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