Y1771
Yeast Synthetic Drop-out Medium Supplements
without uracil, leucine, and tryptophan
Synonym(s):
Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements
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About This Item
Recommended Products
Quality Level
form
powder
technique(s)
transformation: suitable
application(s)
microbiology
storage temp.
room temp
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Application
Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:
- the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
- Yarrowia lipolytica PO1f strain positive transformants
- CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Other Notes
Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.
Preparation Note
Dissolve 1.46 g of supplement per liter of medium.
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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