X1875
Xanthine Oxidase from bovine milk
Grade I, ammonium sulfate suspension, ≥0.4 units/mg protein
Synonym(s):
XOD, Xanthine:oxygen oxidoreductase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
CAS Number:
Enzyme Commission number:
1.17.3.2.
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
type
Grade I
form
ammonium sulfate suspension
specific activity
≥0.4 units/mg protein
foreign activity
uricase ≤0.5%
shipped in
wet ice
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
General description
Xanthine Oxidase (XOD) is a metal flavoprotein. It has flavin adenine dinucleotide (FAD), molybdenum and iron in the ratio 2:2:8. This homodimer has a molecule weight of 290kDa. It is a member of the molybdenum-protein family. This enzyme consists of two separated substrate-binding sites.
Formerly E.C. 1.1.3.22
Application
Xanthine Oxidase from bovine milk has been used:
- in the preparation of xanthine oxidase (XO) solution for 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide (DEPMPO)-spin trapping assay
- in in vitro XO assay for screening Vietnamese medicinal plants for XO inhibitory activity
- as a standard to determine XO activity
- as a standard to test the synergistic effect of docosahexaenoic acid (DHA)
Biochem/physiol Actions
Hydroxylation of hypoxanthine to xanthine and xanthine to uric acid is catalyzed by xanthinebb oxidase (XO) enzyme.
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.
Xanthine oxidase was shown to be involved in the reduction of cytochrome c by the generation of superoxide anions following the oxidation of xanthine. These free radicals are responsible for reducing cytochrome c.
Unit Definition
One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.
Physical form
Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate
Analysis Note
Protein determined by biuret
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Methods of Enzymatic Analysis (2012)
Antioxidant properties of propofol and erythropoietin after closed head injury in rats
Ozturk E, et al.
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 29, 922-927 (2005)
Xanthine oxidase inhibitors from Archidendron clypearia (Jack.) I.C. Nielsen: Results from systematic screening of Vietnamese medicinal plants
Duong NT, et al.
Asian Pacific Journal of Tropical Medicine, 10(6), 549-556 (2017)
Kurt Warnhoff et al.
Genes & development, 35(3-4), 212-217 (2021-01-16)
The molybdenum cofactor (Moco) is a 520-Da prosthetic group that is synthesized in all domains of life. In animals, four oxidases (among them sulfite oxidase) use Moco as a prosthetic group. Moco is essential in animals; humans with mutations in
M F Barroso et al.
Biosensors & bioelectronics, 26(9), 3748-3754 (2011-04-09)
In this paper, a biosensor based on a glassy carbon electrode (GCE) was used for the evaluation of the total antioxidant capacity (TAC) of flavours and flavoured waters. This biosensor was constructed by immobilising purine bases, guanine and adenine, on
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service