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W2394

Sigma-Aldrich

Anti-WIPI-1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ATG18, Anti-WD repeat domain phosphoinositide interacting 1, Anti-WD40 repeat protein Interacting with phosphoInositides of 49kDa, Anti-WIPI-1 alpha, Anti-WIPI49

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~49 kDa

species reactivity

human, rat, mouse

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of mouse 3T3 or at NRK cells
western blot: 2.5-5.0 μg/mL using whole extract of G-361 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... WIPI1(55062)
mouse ... Wipi1(52639)
rat ... Wipi1(303630)

General description

WIPI-1, the mammalian orthologue of Atg18 in S. cerevisiae and A. thaliana, is a member of the WIPI subfamily of WD-repeat proteins. It has a 7-bladed propeller structure and contains a conserved motif for interaction with phospholipids. WIPI-1 is ubiquitously expressed in normal human tissues with highest levels in skeletal muscle, heart and testis. It is aberrantly expressed in human cancer.

Immunogen

synthetic peptide corresponding to amino acids 406-421 of human WIPI-1, conjugated to KLH. The corresponding sequence is identical in mouse and rat.

Application

Anti-WIPI-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

WIPI-1 has essential biological functions including signal transduction, transcription regulation and apoptosis. WD-repeat proteins regulate the assembly of multiprotein complexes by presenting a β-propeller platform for simultaneous and reversible protein-protein interactions. WIPI-1 is linked to starvation-induced autophagy.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Autophagosome formation in mammalian cells
Mizushima N, et al.
Cell Structure and Function, 27(6), 421-429 (2002)
WIPI-1alpha(WIPI49), a member of the novel 7-bladed WIPI protein family, is aberrantly expressed in human cancer and is linked to starvation-induced autophagy
Proikas-Cezanne T, et al.
Oncogene, 23(58), 9314-9314 (2004)
Maria Giovanna De Leo et al.
Autophagy, 17(11), 3644-3670 (2021-03-10)
Autophagosome formation requires PROPPIN/WIPI proteins and monophosphorylated phosphoinositides, such as phosphatidylinositol-3-phosphate (PtdIns3P) or PtdIns5P. This process occurs in association with mammalian endosomes, where the PROPPIN WIPI1 has additional, undefined roles in vesicular traffic. To explore whether these functions are interconnected
Thibault Courtellemont et al.
The EMBO journal, 41(10), e109646-e109646 (2022-04-26)
Endo-lysosomal compartments exchange proteins by fusing, fissioning, and through endosomal transport carriers. Thereby, they sort many plasma membrane receptors and transporters and control cellular signaling and metabolism. How the membrane fission events are catalyzed is poorly understood. Here, we identify

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