Skip to Content
Merck
All Photos(1)

Documents

SRP3161

Sigma-Aldrich

sRANKL human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

ODF (Osteoclast differentiation factor), TNFSF11, TRANCE (TNF-related activation-induced cytokine), soluble Receptor Activator of NFkB Ligand

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

10.0-25.0 ng/mL ED50

mol wt

20.0 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

suitability

suitable for molecular biology

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNFSF11(8600)

General description

Research area: Cell signaling. TRANCE (TNF-related activation-induced cytokine), also known as sRANKL (soluble receptor activator of nuclear factor K-B ligand), is encoded by the gene mapped to human chromosome 13q14.11. sRANKL and RANK (receptor activator of nuclear factors-KB) are members of the tumor necrosis factor (TNF) superfamily of ligands and receptors that play an important role in the regulation of specific immunity and bone turnover. RANKL is expressed in a variety of cells including osteoblasts, fibroblasts, activated T-cells and bone marrow stromal cells. Recombinant human sRANKL is a 20kDa polypeptide comprising the TNF homologous region of RANKL (176 amino acid residues).

Application

sRANKL has been used to initiate differentiation of osteoclasts.

Biochem/physiol Actions

RANKL (receptor activator of nuclear factor K-B ligand) is a potent stimulator of bone resorption. Aberrations in RANK (Receptor activator of nuclear factors-κB) and RANKL (Receptor activator of nuclear factors-κB ligand) genes have been associated with osteoporosis, chronic inflammatory arthritis, the osteolytic bony metastasis of malignancies, and reduced bone mineral density in patients with ankylosing spondylitis. RANK (receptor) was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T-cell proliferation in a mixed lymphocyte reaction, to promote the survival of RANK+ T cells, and to regulate T-cell-dependent immune response. RANKL is also capable of interacting with a decoy receptor called OPG (osteoprotegerin). Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage.

Sequence

MEKAMVDGSW LDLAKRSKLE AQPFAHLTIN ATDIPSGSHK VSLSSWYHDR GWAKISNMTF SNGKLIVNQD GFYYLYANIC FRHHETSGDL ATEYLQLMVY VTKTSIKIPS SHTLMKGGST KYWSGNSEFH FYSINVGGFF KLRSGEEISI EVSNPSLLDP DQDATYFGAF KVRDID

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.6 + 75 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases.
Zhu J
The Journal of Biological Chemistry, 282, 26656-26664 (2007)
Elevated serum levels of soluble receptor activator of nuclear factors-kappaB ligand (sRANKL) and reduced bone mineral density in patients with ankylosing spondylitis (AS).
Kim HR
Rheumatology (Oxford, England), 45, 1197-1200 (2006)
The role of osteoprotegerin and receptor activator of nuclear factor KB ligand in the pathogenesis and treatment of rheumatoid arthritis.
Hofbauer L C and Armin E H
Arthritis & Rheumatology (Hoboken, N.J.), 44(2), 253-259 (2001)
Cost-effective analysis of candidate genes using htSNPs: a staged approach.
Lowe C E, et al.
Genes and Immunity, 5(4), 301-301 (2004)
Interactions of tumor necrosis factor (TNF) and TNF receptor family members in the mouse and human.
Bossen C, et al.
The Journal of Biological Chemistry, 281(20), 13964-13971 (2006)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service