SRP3042

FGF-acidic human

Animal-component free, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

• Synonym(s): ECGF-beta, FGF-1, Fibroblast Growth Factor-acidic, HBGF-1
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥95% (HPLC); ≥95% (SDS-PAGE)
• form: lyophilized
• potency: ≥2x10^6 units/mg
• mol wt: 15.8 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P05230
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... FGF1(2246)

Description

General description

FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

Biochem/physiol Actions

FGF-acidic is one of 23 known members of the FGF family. Recombinant human FGF-acidic is a 15.8 kDa protein consisting of 140 amino acid residues.

Sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 8.0 + 100 mM NaCl.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of <0.5 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable