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Key Documents

N9162

Sigma-Aldrich

Neocarzinostatin

from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), solution

Synonym(s):

Holoneocarzinostatin, NCS, NSC-69856, Zinostatin

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Neocarzinostatin from Streptomyces carzinostaticus, ≥90% (SDS-PAGE), ~0.5 mg/mL

Quality Level

Assay

≥90% (SDS-PAGE)

form

solution

concentration

~0.5 mg/mL

antibiotic activity spectrum

neoplastics

Mode of action

DNA synthesis | interferes

shipped in

wet ice

storage temp.

2-8°C

InChI

1S/C35H35NO12/c1-16-12-19(42-4)14-22-20(16)8-9-23(37)27(22)32(40)45-24-13-18-10-11-35(26-15-43-34(41)46-26)25(48-35)7-5-6-21(18)31(24)47-33-28(36-3)30(39)29(38)17(2)44-33/h8-9,12-14,17,21,24-26,28-31,33,36-39H,6,15H2,1-4H3/t17-,21?,24-,25-,26-,28-,29+,30-,31-,33-,35+/m1/s1

InChI key

BLXZMHNVKCEIJX-PNKAXBHOSA-N

Application

Neocarzinostatin from Streptomyces carzinostaticus has been used:
  • as a DNA damaging agent to treat human fibroblast GM0637 cells
  • as a radiomimetic antibiotic to induce DNA damage
  • in combination with Vγ2Vδ2 T cells to determine the cytotoxicity in ovarian cancer cells

Biochem/physiol Actions

Neocarzinostatin is a protein-small molecule complex composed of an enediyne chromophore tightly bound to a 113 amino acid single chain protein. The complex possesses antiproliferative and antitumor activity. The chromophore is the active compound, which is responsible for DNA cleavage; while the apoprotein stabilizes and regulates the availability of the labile chromophore. NCS chromophore is bound non-covalently in a cleft of the binding protein and is dissociable. Upon addition of a thiol, the chromophore forms a highly reactive biradical species that can induce sequence-specific single and double strand breaks in DNA. Neocarzinostatin inhibits DNA synthesis and possesses antitumor activity in various human and animal tumors. NCS inhibits cellular proliferation by inducing G2 cell cycle arrest and apoptosis in both human papillomavirus (HPV) positive and negative cell lines.
Neocarzinostatin is a radiomimetic antibiotic substance.

Physical form

Supplied as 100 μg (~9 nmoles) in a solution containing 20 mM MES buffer, pH 5.5.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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