Skip to Content
Merck
All Photos(1)

Key Documents

MAK506

Sigma-Aldrich

Peroxidase Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Peroxidase Enzyme Activity Kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352204
NACRES:
NA.84

Quality Level

detection method

colorimetric
fluorometric

relevant disease(s)

cancer; cardiovascular diseases; neurological disorders

shipped in

wet ice

storage temp.

−20°C

General description

Peroxidase catalyze the following oxidationreduction reactions: ROOR′ + electron donor (2 e-) + 2H+ → ROH + R′OH For many peroxidases the optimal substrate is hydrogen peroxide (H2O2), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi, and bacteria (cytochrome-c peroxidase like enzymes such as horseradish peroxidase).

Features and Benefits

  • Linear detection range: 2 to 50 U/L for the colorimetric assay and 0.1 to 5 U/L for the fluorometric assay.
  • Compatible with high-throughput handling systems.

Suitability

The kit is suitable for peroxidase activity determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Principle

The Peroxidase Assay Kit uses H2O2 and an electron donor dye that forms resorufin during the peroxidase reaction. The optical density at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is a direct measure of the enzyme activity.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service