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M7569

Sigma-Aldrich

Monoclonal Anti-MTA2 antibody produced in mouse

clone MTA2-276, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Metasisis-associated Gene

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MTA2-276, monoclonal

form

buffered aqueous solution

mol wt

antigen ~73 kDa

species reactivity

mouse, human, bovine, rat, hamster, canine, monkey

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using 293T total cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MTA2(9219)
mouse ... Mta2(23942)
rat ... Mta2(361724)

General description

Metastasis associated 1 family member 2 (MTA2) is part of the nucleosome remodeling and histone deacetylation complex. This 668 amino acid protein possesses four different domains.
Monoclonal Anti-MTA2 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MTA2, conjugated to KLH. Human metastasis-associated protein 2 (MTA2) shares 58% homology to human MTA1 and MTA3 proteins, the C-terminus being more divergent than the N-terminus. MTA2 comprises N-terminal bromo-adjacent homology (BAH) and MTA1 homology 2 (ELM2) domain, SANTand zinc finger (ZnF) domain. It also has the nuclear localization region at C-terminus.

Immunogen

synthetic peptide corresponding to amino acids 626-641of human MTA2, conjugated to KLH.

Application

Monoclonal Anti-MTA2 antibody produced in mouse has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoprecipitation
  • immunocytochemistry
  • Western blotting
  • antibody microarray

Biochem/physiol Actions

Human metastasis-associated protein 2 (MTA2) interacts with the histone deacetylase-1 HDAC1 and within the nuclear remodeling and deacetylation complexes ATP-dependent helicase -nucleosome remodelling and histone deacetylation (Mi2/NuRD), suggests that these proteins are involved in transcriptional repression. In the p53 pathway, MTA2/MTA1L1 is better known as protein in the deacetylase complexes p53 target protein in the deacetylase complexes (PID). It inhibits p53-dependent transcriptional activation and its growth arrest and apoptosis functionality. showing that deacetylation and functional interaction by the PID/MTA2/MTA1L1 associated nucleosome remodeling deacetylase (NuRD) complex may represent an important pathway to regulated p53 function.
Metastasis associated 1 family member 2 (MTA2) has been associated with tumor progression and metastasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Validation of a novel shotgun proteomic workflow for the discovery of protein-protein interactions: Focus on ZNF521
Bernaudo F, et al.
Journal of Proteome Research, 14(4), 1888-1899 (2015)
Overexpression of MTA1 inhibits the metastatic ability of ZR-75-30 cells in vitro by promoting MTA2 degradation
Zhang L, et al.
Cell communication and signaling : CCS, 17(1), 4-4 (2019)
Epigenetic reactivation of LINE-1 retrotransposon disrupts Nu RD corepressor functions and induces oncogenic transformation in human bronchial epithelial cells
Bojang Jr P and Ramos KS
Molecular Oncology, 12(8), 1342-1357 (2018)
Deacetylation of p53 modulates its effect on cell growth and apoptosis
Luo J, et al.
Nature, 408(6810), 377-377 (2000)
Kyle R Covington et al.
Cancer metastasis reviews, 33(4), 921-928 (2014-11-15)
Metastasis is the ultimate cause of death for most cancer patients. While many mechanisms have been delineated for regulation of growth and tumor initiation of the primary tumor, very little is known about the process of metastasis. Metastasis requires dynamic

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