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M1633

Sigma-Aldrich

4-Methylumbelliferyl β-D-galactopyranoside

fluorogenic, ≥99% (TLC), powder

Synonym(s):

MUG, MUGA

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About This Item

Empirical Formula (Hill Notation):
C16H18O8
CAS Number:
6160-78-7
Molecular Weight:
338.31
Beilstein:
94673
EC Number:
228-185-5
MDL number:
MFCD00036773
UNSPSC Code:
12352204
PubChem Substance ID:
24896676
NACRES:
NA.32

product name

4-Methylumbelliferyl β-D-galactopyranoside, ≥99% (TLC)

Assay

≥99% (TLC)

form

powder

solubility

pyridine: 10 mg/mL, clear, colorless to faintly yellow

storage temp.

−20°C

SMILES string

CC1=CC(=O)Oc2cc(O[C@@H]3O[C@H](CO)[C@H](O)[C@H](O)[C@H]3O)ccc12

InChI

1S/C16H18O8/c1-7-4-12(18)23-10-5-8(2-3-9(7)10)22-16-15(21)14(20)13(19)11(6-17)24-16/h2-5,11,13-17,19-21H,6H2,1H3/t11-,13+,14+,15-,16-/m1/s1

InChI key

YUDPTGPSBJVHCN-DZQJYWQESA-N

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General description

4-Methylumbelliferyl β-D-galactopyranoside (MUG) is a UV-absorbing dye that serves as a fluorescent substrate for β-galactosidase in a planar yeast estrogen screen (pYES) for the detection of estrogen active compounds.

Application

4-Methylumbelliferyl β-D-galactopyranoside has been used as a substrate in β-galactosidase activity assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Frontiers in plant science, 8, 1447-1447 (2017-09-06)
Inducible gene expression has emerged as a powerful tool for plant functional genomics. The estrogen receptor-based, chemical-inducible system XVE has been used in many plant species, but the limited systemic movement of inducer β-estradiol in transgenic rice plants has prohibited
Dinah Schick et al.
Journal of chromatography. A, 1497, 155-163 (2017-04-01)
For the planar yeast estrogen screen (pYES), 4-methylumbelliferyl-β-d-galactopyranoside was generally employed as substrate, delivering blue fluorescing 4-methylumbelliferone after enzymatic cleavage by the YES reporter β-d-galactosidase as the positive signal for the presence of estrogen active compounds (EAC). As environmental samples
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