H8142

Hybridoma Enhancing Supplement from murine macrophage-like cell line

liquid, sterile-filtered, suitable for hybridoma

• Synonym(s): Conditioned Medium from murine macrophage-like cell line
• UNSPSC Code: 12352205

Properties

• sterility: sterile-filtered
• Quality Level: 200
• form: liquid
• technique(s): cell culture | hybridoma: suitable
• impurities: Mycoplasma, tested; endotoxin, tested
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Use at a concentration of 10% in complete medium containing FBS (10%) or CPRS-3. Optimal conditions must be determined for specific applications.

Other Notes

Conditioned medium from a proprietary murine macrophage-like cell line grown in IMDM with 2% FBS.

Caution

Avoid repeated freezing and thawing. If entire contents are not used, store frozen in working aliquots. Precipitation may occur when stored. This is not detrimental to the product and may be reversed when warmed to 37 °C.

Analysis Note

Cell line viral testing:
Murine Antibody Production (map) testing for Sendai, MHV, PVM, Reo3, GDVII, Ectomelia, MVM, Polyoma, LDMV, M. Ad, EDIM, MCMV, LCM, Hantaan.
BVD virus by fluorescent antibody stain

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves