GE28-9782-45

HiScreen^™, DEAE Fast Flow

Cytiva 28-9782-45

• UNSPSC Code: 47101511
• NACRES: NA.56

Properties

• ligand: diethylaminoethyl
• description: Ion Exchanger Type (value)
• form: prepacked column
• crosslinking: cross-linked
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 28-9782-45
• storage condition: (20% Ethanol)
• parameter: 1.5 bar (22 psi) (Over the Packed Bed During Operation); 22 psi
• technique(s): liquid chromatography (LC): suitable
• bed size: 7.7 mm × 100 mm
• bed volume: 4.7 mL
• column I.D.: 7.7 mm
• matrix: 6% cross-linked agarose
• matrix active group: N⁺(C₂H₅)₂H functional group
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning: 2-14
• working range: 2-12
• suitability: suitable for bioprocess medium
• separation technique: anion exchange

Description

General description

HiScreen^™ DEAE FF columns are prepacked with DEAE Sepharose Fast Flow, a weak anion exchanger, and are part of the process development platform available from Cytiva. The columns are an excellent choice for method optimization and parameter screening.

Application

The small column volume of 4.7 mL and bed height of 10 cm make HiScreen^™ columns excellent tools for method optimization, parameter screening, robustness testing, and convenient scale-up. Process fluid velocities can be applied, since the 10 cm bed height gives enough residence time and the results can then serve as basis for linear process scale-up.

If necessary, two columns can easily be connected in series to give a bed height of 20 cm.

Media prepacked in HiScreen^™ IEX FF columns are also available in other formats and as bulk packs, making it possible to use the same media for development work, pilot studies, and routine production.

Features and Benefits

• DEAE Sepharose Fast FLow is a weak anion exchanger and IEX Sepharose^™ Fast FLow medium are well-proven, industrial standards for ion exchange chromatography.
• Prepacked, ready-to-use columns for convenient process development optimization.
• 10 cm bed height of HiScreen^™ columns is designed to allow method optimization and parameter screening.
• Easily connected in series to achieve 20 cm bed height.
• Small bed volume gives fast results and minimal sample/buffer consumption.
• Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiScreen is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids