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G1N9604

Sigma-Aldrich

GenElute™ 96 Well Tissue Genomic DNA Purification Kit

4 x 96 well plate, sufficient for 4 96-well plate purifications

Synonym(s):

High Throughput Purification, Tissue Genomic DNA

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

usage

sufficient for 4 96-well plate purifications

quality

4 x 96 well plate

storage temp.

15-25°C

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General description

GenElute 96 Well Tissue Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations, and hazardous compounds such as phenol and chloroform.

The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.

The purified genomic DNA is ready for use in downstream applications such as PCR, Southern blotting, or any kind of enzymatic reaction.

Application

The purified genomic DNA is ready for use in downstream applications such as:
  • PCR
  • Southern blots
  • cloning
  • restriction digest
  • enzymatic reactions

Features and Benefits

  • Starting material: ≤ 20 mg tissue total (mouse, rat or whole sample) or ≤ 1 X 106 cultured cells or bacteria per well
  • Average Expected yield: 10 - 20 μg/well (bacteria: 1.5 - 2.5 μg/well)
  • Elution volume: 100 μl
  • Time required: 70 min/plate
  • Innovative wash plate minimizes risk of cross-contamination
  • Processing possible under vacuum or centrifugation
  • Suitable for manual and automated processing

Principle

The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol, which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane, several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class Code

3 - Flammable liquids

Flash Point(F)

75.2 °F

Flash Point(C)

24 °C

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Certificates of Analysis (COA)

Lot/Batch Number
071M0758

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Marta Lukačišinová et al.
Nature communications, 11(1), 3105-3105 (2020-06-21)
Genetic perturbations that affect bacterial resistance to antibiotics have been characterized genome-wide, but how do such perturbations interact with subsequent evolutionary adaptation to the drug? Here, we show that strong epistasis between resistance mutations and systematically identified genes can be

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