Skip to Content
Merck
All Photos(1)

Key Documents

F6682

Sigma-Aldrich

Anti-Filamin antibody,Mouse monoclonal

clone PM6/317, purified from hybridoma cell culture

Synonym(s):

Anti-ABP-280, Anti-ABPX, Anti-CSBS, Anti-CVD1, Anti-FGS2, Anti-FLN, Anti-FLN-A, Anti-FLN1, Anti-FMD, Anti-MNS, Anti-NHBP, Anti-OPD, Anti-OPD1, Anti-OPD2, Anti-XLVD, Anti-XMVD

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

PM6/317, monoclonal

form

buffered aqueous solution

mol wt

antigen ~190 kDa

species reactivity

human, monkey, hamster, bovine, canine

concentration

~1.0 mg/mL

technique(s)

indirect immunofluorescence: suitable
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole extract of human Jurkat or HEK-293Tcells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... FLNA(2316)

General description

Filamin, also known as actin-binding protein, is a large flexible V-shaped actin cross-linking cytoplasmic protein present in vertebrates of both muscle and non-muscle cells. It has actin-binding domains at the ends of each arm. It is a polypeptide with molecular weight of approximately 250 kDa and forms dimers in solution. It has a large flexible actin cross-linking protein present in vertebrates in both the muscle and non-muscle cells. The name filamins refers to its filamentous colocalization with actin stress fibers. It has an actin binding domain and a homodimerization domain. Native form comprises of two subunits with one actin-binding site.
Monoclonal Anti-Filamin (mouse IgG1 isotype) is derived from the hybridoma PM6/317 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human platelet membranes.

Immunogen

human platelet membranes

Application

Monoclonal Anti-Filamin antibody is suitable for indirect immunofluorescence and western blot (chemiluminescent) at a concentration of 0.5-1.0μg/mL using whole extract of human Jurkat or HEK-293Tcells.
Monoclonal Anti-Filamin antibody produced in mouse has been used in:
  • immunoblotting
  • immunofluorescent staining
  • immunoprecipitation

Biochem/physiol Actions

Filamin has been primarily identified from smooth muscle but it has also found in macrophages, neutrophils, platelets and other cell types. It is associated in regulation of the cytoskeletal network by cross-linking actin and linking cell membrane to the cytoskeleton. It is also linked in regulating intracellular signaling pathways, responsible for skeletal development. It binds to the ring zinc finger domain of tumor necrosis factor (TNF) and receptor-associated factor 2 (TRAF2). Filamin cross-links with actin and promotes its aggregation and gelation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Takanori Chiba et al.
Scientific reports, 6, 28383-28383 (2016-06-22)
Cell competition is a cell-cell interaction by which a cell compares its fitness to that of neighboring cells. The cell with the relatively lower fitness level is the "loser" and actively eliminated, while the cell with the relatively higher fitness
Structure and Organization of Actin Filaments
Cooper GM et al.
Cell (2000)
Sayaka Yamamoto et al.
Molecular biology of the cell, 27(3), 491-499 (2015-12-04)
At the initial step of carcinogenesis, transformation occurs in single cells within epithelia, where the newly emerging transformed cells are surrounded by normal epithelial cells. A recent study revealed that normal epithelial cells have an ability to sense and actively
ADAM-like Decysin-1 (ADAMDEC1) is a positive regulator of Epithelial Defense Against Cancer (EDAC) that promotes apical extrusion of RasV12-transformed cells
Yako Y, et al.
Scientific reports, 8(1), 9639-9639 (2018)
Jie Lu et al.
Human molecular genetics, 16(14), 1661-1675 (2007-05-19)
Filamin B (FLNB) is a cytoplasmic protein that regulates the cytoskeletal network by cross-linking actin, linking cell membrane to the cytoskeleton and regulating intracellular signaling pathways responsible for skeletal development (Stossel, T.P., Condeelis, J., Cooley, L., Hartwig, J.H., Noegel, A.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service