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form
liquid
usage
1000 uses (0.5 mg size)
200 uses (0.1 mg size)
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Components
Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
Other Notes
Contains 22 fragments, 8–587 bp
For optimal resolution, the recommended agarose gel concentration is 2.5.
Preparation Note
Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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