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CAR, ML human cell line

94082207, IHW Number: 9108

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About This Item

UNSPSC Code:
41106514

Pricing and availability is not currently available.

biological source

human blood

ethnicity

Not specified

gender

not specified

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

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This Item
HPA018265HPA070710HPA038499
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

UniProt accession no.

Q99470

UniProt accession no.

O95926

UniProt accession no.

O95926

UniProt accession no.

-

General description

Consanguineous: No
Homozygous: No

Cell Line Description

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA Profile

Cw: 401
HLA DRB1: 0101 1401
HLA DRB3: 202
HLA DQA1: 101
HLA DQB1: 501
HLA DPB1: 0401 0402

Serological Profile

HLA A: 2
HLA C: 4 3
HLA B: 35 55
Bw4/bw6: 6

Culture Medium

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Subculture Routine

Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

Certificates of Analysis (COA)

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