C-28053
Dendritic Cell Generation Medium
Basal Medium, 250 ml
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352207
NACRES:
NA.71
Recommended Products
form
liquid
packaging
pkg of 250 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
DC Base Medium (supplements sold separately)
Application
The Dendritic Cell (DC) Base Medium is a user-customizable version of the DC Generation Media that does not include cytokines. It must be adequately supplemented by the user for the generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes.Note: DC Base Medium must be supplemented with additional cytokines. For freshly isolated cells, the Monocyte Attachment Medium (C-28051) is also needed.Recommended for:Human Monocytes (hMo), freshly isolated and cryopreservedHuman Mononuclear Cells (hMNC), freshly isolated
Quality
All lots of PromoCell DC Media are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell DC Media is tested for the ability to support DC maturation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage and Stability
Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
Subculture Routine
Click here for more information.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service