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A3400

Sigma-Aldrich

Anti-Human IgA (α-chain specific)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Human IgA (α-chain specific)−AP

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:7,000-1:21,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Goat anti-Human IgA (α-chain specific)-alkaline phosphatase antibody is specific for human IgA when tested against human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins.

Immunogen

human IgA

Application

Goat anti-Human IgA (α-chain specific)-alkaline phosphatase antibody has been used to quantitate serum antibody to H. pylori using ELISA techniques.
Milk samples from 10 mothers were probed for bound milk IgA by western blot using alkaline-phosphatase goat anti-human IgA antibody.Elisa Assays were performed using alkaline phosphatase conjugated gaot anti-human IgA chain specific antibody (1:1000) to detect IgA immunoglobulins in serum and seminal plasma samples from infertile men.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 50% glycerol , 1 mM MgCl2, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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P Ramaprasad et al.
Leprosy review, 68(4), 301-315 (1998-03-21)
Recent advances in treatment have achieved a large drop in the prevalence of active leprosy cases, but the incidence is at best decreasing slowly. Most people within leprosy-endemic populations have been exposed to Mycobacterium leprae, but few develop disease and
Sumedha Sharma et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 54(3), 1751-1759 (2023-05-18)
Non-sputum-based biomarker assay is urgently required as per WHO's target product pipeline for diagnosis of tuberculosis. Therefore, the current study was designed to evaluate the utility of previously identified proteins, encoded by in vivo expressed mycobacterial transcripts in pulmonary tuberculosis
Terry M Nolan et al.
EBioMedicine, 98, 104878-104878 (2023-11-29)
SARS-CoV-2 booster vaccination should ideally enhance protection against variants and minimise immune imprinting. This Phase I trial evaluated two vaccines targeting SARS-CoV-2 beta-variant receptor-binding domain (RBD): a recombinant dimeric RBD-human IgG1 Fc-fusion protein, and an mRNA encoding a membrane-anchored RBD.
R D Leunk et al.
Journal of clinical microbiology, 28(6), 1181-1184 (1990-06-01)
Gastrointestinal disease and colonization by Helicobacter pylori were determined in 36 asymptomatic volunteers and 30 symptomatic individuals undergoing endoscopy and biopsy. Serum antibody immunoglobulin G (IgG) and IgA to H. pylori were measured by enzyme-linked immunosorbent assay. Serum antibody to
S Ali et al.
The British journal of dermatology, 175(1), 113-121 (2016-01-23)
The use of saliva for the diagnosis of pemphigus vulgaris (PV) by enzyme-linked immunosorbent assay (ELISA) using desmoglein (Dsg)3 antigen has not been extensively documented, nor has the detection of serum IgA antibodies to Dsg3. (i) To establish whether whole

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