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45245

Sigma-Aldrich

Eosin 5-isothiocyanate

≥95.0% (UV)

Synonym(s):

EITC

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About This Item

Empirical Formula (Hill Notation):
C21H7Br4NO5S
CAS Number:
60520-47-0
Molecular Weight:
704.96
MDL number:
MFCD00082502
UNSPSC Code:
12352200
PubChem Substance ID:
57650268

Assay

≥95.0% (UV)

fluorescence

λex 521; λem 544 (pH 9.0)

storage temp.

2-8°C

SMILES string

OC(=O)c1cc(ccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c(O)c(Br)cc24)N=C=S

InChI

1S/C21H7Br4NO5S/c22-12-4-10-14(8-2-1-7(26-6-32)3-9(8)21(29)30)11-5-13(23)18(28)16(25)20(11)31-19(10)15(24)17(12)27/h1-5,27H,(H,29,30)

InChI key

MCXYFYWNOWPDMA-UHFFFAOYSA-N

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Application

Eosin 5-isothiocyanate (EITC) is a fluorescence probe. EITC tagged molecules may be used together with fluorescein-5′-isothiocyanate (FITC) tagged molecules in fluoresce resonance energy transfer (FRET) based assays.

Other Notes

Acceptor fluorophore. Measurement of rotational diffusion of proteins; In fluorescence energy transfer studies

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317 - H334

Precautionary Statements

P261 - P280 - P342 + P311

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T Chiba et al.
Biochimica et biophysica acta, 858(1), 107-117 (1986-06-13)
The amino-acid residues of band 3 protein taking part in the ionic interaction with an anion-transport inhibitor, eosin 5-isothiocyanate (EITC), were determined by pH titration. The plots of the absorbance of EITC-ghost system against pH reveal five equilibria, at pH
T J Deerinck et al.
The Journal of cell biology, 126(4), 901-910 (1994-08-01)
A simple method is described for high-resolution light and electron microscopic immunolocalization of proteins in cells and tissues by immunofluorescence and subsequent photooxidation of diaminobenzidine tetrahydrochloride into an insoluble osmiophilic polymer. By using eosin as the fluorescent marker, a substantial
S Papp et al.
Biophysical journal, 51(2), 205-220 (1987-02-01)
Ca2+-ATPase molecules were labeled in intact sarcoplasmic reticulum (SR) vesicles, sequentially with a donor fluorophore, fluorescein-5'-isothiocyanate (FITC), and with an acceptor fluorophore, eosin-5'-isothiocyanate (EITC), each at a mole ratio of 0.25-0.5 mol/mol of ATPase. The resonance energy transfer was determined
C Gatto et al.
The American journal of physiology, 264(6 Pt 1), C1577-C1586 (1993-06-01)
This paper addresses the mechanism of inhibition of the plasma membrane Ca pump by fluorescein analogues and their isothiocyanate derivatives. Eosin (i.e., tetrabromofluorescein) was found to be one of the most potent reversible inhibitors of the erythrocyte Ca pump [half-maximal
R. Wagner et al.
Febs Letters, 230, 109-109 (1988)
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