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12138C

Sigma-Aldrich

Bovine Calf Serum

Iron-Fortified, USA origin, from formula-fed bovine calves, sterile-filtered, suitable for cell culture

Synonym(s):

Calf Serum, BCS, bovine calf sera, calf sera, calf serum

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About This Item

MDL number:
MFCD00243108
UNSPSC Code:
12352207
NACRES:
NA.71

biological source

bovine (calf) (formula-fed)

Quality Level

500

sterility

sterile-filtered

composition

hemoglobin, ≤35 mg/dL

origin

USA origin

technique(s)

cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

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Application

Iron-supplemented bovine serum as an alternative to fetal bovine serum in the CHO/HGPRT mutation assay, Oberly, et. al. Development of a more rapid, reduced serum culture system for Caco-2 monolayers and application to the biopharmaceutics classification system. Lentz et. al.

Testing Method

Endotoxin tested

Preparation Note

Each lot is supplemented to a constant iron concentration.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D R Dahling et al.
Journal of virological methods, 27(3), 287-294 (1990-03-01)
Two studies comparing sewage-isolated and laboratory stock viruses were conducted to determine if alternative forms of serum or serum extenders could be used in place of fetal bovine serum without a significant loss of viral titer. In the first study
T J Oberly et al.
Mutation research, 244(2), 105-109 (1990-06-01)
Iron-supplemented bovine calf serum (ICS) was found to be a viable alternative to fetal bovine serum (FBS) in the growth promotion and cloning efficiency of Chinese hamster ovary (CHO) cells that are used in the HGPRT mutation assay. Suspension cultures
K A Lentz et al.
International journal of pharmaceutics, 200(1), 41-51 (2000-06-14)
The objectives were: (1) to develop a more rapid, reduced serum culture system for Caco-2 monolayers, relative to the traditional 21-day, 10% fetal bovine serum (FBS) system; and (2) to determine the biopharmaceutical drug classification of an oral therapeutic agent
Wei Wang et al.
PloS one, 10(4), e0119005-e0119005 (2015-04-24)
Conjugated linoleic acid (CLA) reduces adiposity in human and mouse adipocytes. This outcome is achieved through a variety of biological responses including increased energy expenditure and fatty acid oxidation, increased inflammation, repression of fatty acid biosynthesis, attenuated glucose transport, and

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