02744
Atto 655-Streptavidin
BioReagent, suitable for fluorescence
Synonym(s):
Streptavidin-Atto 655
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About This Item
Recommended Products
product line
BioReagent
Quality Level
form
powder
manufacturer/tradename
ATTO-TEC GmbH
solubility
DMF: soluble
DMSO: soluble
acetonitrile: soluble
fluorescence
λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0
suitability
suitable for fluorescence
suitable for gel electrophoresis
storage temp.
−20°C
Application
Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.
Analysis Note
free of unconjugated dye
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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