Skip to Content
Merck
All Photos(4)

Documents

D0531

Sigma-Aldrich

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride

≥90% purity (HPLC), powder

Synonym(s):

DTAF

Sign Into View Organizational & Contract Pricing


About This Item

Empirical Formula (Hill Notation):
C23H12Cl2N4O5 · HCl
CAS Number:
Molecular Weight:
531.73
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride, ≥90% (HPLC)

Quality Level

Assay

≥90% (HPLC)

form

powder

color

yellow to orange

solubility

methanol: 20 mg/mL, clear to slightly hazy, yellow to orange

fluorescence

λex 495 nm; λem 516 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1

InChI

1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H

InChI key

CEKSHCAIHGNWBK-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea). It has also been used to label bacteria (Vibrio spp.).
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.
A fluorescein derivative which reacts with amino groups and N-terminal amino acids of proteins at neutral pH.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

A mechanism for the synergistic gelation properties of gelatin B and xanthan gum aqueous mixtures
Wang CS, et al.
Carbohydrate Polymers, 175, 484-492 (2017)
Bruce L Granger
PloS one, 13(1), e0191194-e0191194 (2018-01-13)
Yeast wall protein 1 (Ywp1) is an abundant glycoprotein of the cell wall of the yeast form of Candida albicans, the most prevalent fungal pathogen of humans. Antibodies that bind to the polypeptide backbone of isolated Ywp1 show little binding
A gelation mechanism for gelatin/polysaccharide aqueous mixtures
Wang CS, et al.
Food Hydrocolloids, 79, 462-472 (2018)
Synergistic gelation of gelatin B with xanthan gum
Wang CS, et al.
Food Hydrocolloids, 60, 374-383 (2016)
Adrián Pérez-Ramos et al.
Frontiers in microbiology, 9, 1684-1684 (2018-08-10)
Lactic acid bacteria synthesize exopolysaccharides (EPS), which could benefit the host's health as immunomodulators. Furthermore, EPS could protect bacteria against gastrointestinal stress, favoring gut colonization, thus protecting the host against pathogenic infections. Pediococcus parvulus 2.6, produces a 2-substituted (1,3)-β-D-glucan and

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service