B0126 is a pH indicator that changes color based on pH levels. It turns blue at pH 4.6 and above, yellow below pH 3.0, and may turn green between pH 4.6 and 3.0. A change to a color not mentioned above or the solution becoming clear indicates the presence of bleach residues.
B0126
Bromophenol Blue
Powder
Synonym(s):
3′,3′′,5′,5′′-Tetrabromophenolsulfonephthalein, Bromophenol Blue (BPB), Bromphenol Blue Sultone Form
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About This Item
Empirical Formula (Hill Notation):
C19H10Br4O5S
CAS Number:
Molecular Weight:
669.96
Beilstein:
61698
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
Product Name
Bromophenol Blue, titration: suitable
Agency
suitable for SM 2310
suitable for SM 2320
Quality Level
form
powder
technique(s)
titration: suitable
color
faint beige to very dark beige
faint orange to very dark orange
visual transition interval
3.0-4.6, yellow-green to blue
mp
273 °C
solubility
methanol: 10 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
InChI
1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key
UDSAIICHUKSCKT-UHFFFAOYSA-N
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General description
Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein. Bromophenol Blue is a pH indicator and a dye appears a strong blue color. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Application
Bromophenol Blue is used as a tracking dye in DNA, RNA (agarose) and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels.It has also been known for its use as a vital stain to probe the blood-brain barrier.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Is it safe to use bleach as a deactivating agent with B0126, considering that bleach is an oxidizing agent?
1 answer-
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