agriculture environmental food and beverages water monitoring microbiology
suitability
selective and differential for Escherichia coli selective and differential for coliforms
Application
Fluorescent method for the detection of E. coli.
LST-MUG broth is a growth media, which is used for the selective detection and differentiation of Escherichia coli in water samples.
Preparation Note
Dissolve 36.5 g in 1 litre distilled water and fill into tubes which can be optionally fitted out with Durham tubes. Sterilize by autoclaving at 121°C for 15 minutes.
Other Notes
Fluorogenic assay for rapid detection of E. coli in chilled and frozen foods.[1]
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Journal - Association of Official Analytical Chemists, 71(3), 589-602 (1988-05-01)
A collaborative study was conducted to compare a proposed LST-MUG method with the AOAC official method for Escherichia coli detection. E. coli produces an enzyme, beta-glucuronidase, which cleaves the substrate, 4-methyl-umbelliferyl-beta-D-glucuronide (MUG), to yield a fluorescent end product. Incorporation of
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