This product is released based on the testing performed by the Biological Stain Commission. There is no specific solubility specification for this product. At the BSC, the product is dissolved at 50 mg in 250 ml of water for one test, and 100 mg of the dye is dissolved in 150 ml of water for the second test, followed by the addition of additional alcohol. When used to stain frozen sections, a 0.5% solution is typically prepared in 20% alcohol. Some procedures call for dissolving at 10 mg/ml, but this amount of dye cannot always be dissolved. Once dissolved, the solution should be relatively stable.This product does not appear to be hygroscopic, and its solubility can vary somewhat from lot to lot.
198161
Toluidine Blue O
certified by the Biological Stain Commission
Synonym(s):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
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About This Item
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47
Pricing and availability is not currently available.
Recommended Products
grade
certified by the Biological Stain Commission
Quality Level
form
powder
composition
Dye content, 80%
color
dark green
λmax
626 nm
ε (extinction coefficient)
≥13000 at 241-247 nm in H2O
≥28000 at 287-293 nm in H2O
≥40000 at 625-635 nm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
InChI
1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
InChI key
GEDVVYWLPUPJJZ-UHFFFAOYSA-N
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General description
A metachromatic dye suitable for a wide variety of histological staining procedures.
Application
Biochem/physiol Actions
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls.[3] TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite.[4] TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries.[5] This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.[6]
Suitability
Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Customers Also Viewed
Photosensitization of in vitro biofilms by toluidine blue O combined with a light-emitting diode.
Zanin IC
European Journal of Oral Sciences, 114(1), 64-69 (2006)
Factors influencing the susceptibility of Gram-negative bacteria to toluidine blue O-mediated lethal photosensitization
komerik N
Journal of Applied Microbiology, 92(4), 618?623-618?623 (2002)
A TRIBASIC STAIN FOR THIN SECTIONS OF PLASTIC-EMBEDDED, OSO4-FIXED TISSUES.
P M GRIMLEY
Stain technology, 39, 229-233 (1964-07-01)
Polychromatic staining of plant cell walls by toluidine blue O
Brien T P O
Protoplasma, 59(2), 368- 373 (1964)
The Effect of Variable Energy Input from a Novel Light Source on the Photoactivated Bactericidal Action of Toluidine Blue O on Streptococcus mutans
Williams J A
Caries Research, 37, 190?193-190?193 (2003)
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Is there a recommendation for dissolving Toluidine Blue (198161-5g lot MKCK0257) at 1 mg/ml in water?
1 answer-
Helpful?
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Could you provide the protocol for using Toluidine Blue for MMA sections?
1 answer-
There are no protocols for using Toluidine Blue O to stain MMA sections.
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