11585550910
Roche
PCR DIG Labeling Mix
solution, suitable for PCR
Synonym(s):
nucleic acid labeling
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41105500
Recommended Products
form
solution
Quality Level
usage
sufficient for 2 x 25 assays (100 ul final reaction volume)
packaging
pkg of 500 μL (2 x 250 μl)
manufacturer/tradename
Roche
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
PCR: suitable
color
colorless
solubility
water: miscible
greener alternative category
, Aligned
storage temp.
−20°C
General description
PCR DIG Labeling Mix is a nucleotide mixture that can be added directly to polymerase chain reactions (PCR) and the digoxigenin (DIG)-labeled nucleotide will be incorporated into the PCR product. Taq DNA polymerase, as well as Tth (Thermus thermophilus) DNA polymerase, can be used for the synthesis of DIG-labeled PCR products. The PCR DIG Labeling Mix can replace the unlabeled nucleotide mix in PCR. 10μl of the PCR DIG Labeling Mix is used in a standard 100μl PCR assay.
When using higher concentrations of DIG-deoxyuridinetriphosphate (dUTP) what is supplied with the PCR DIG Labeling Mix, the yield of the PCR product may be reduced, but however, the label intensity and the molecular weight of the PCR product is increased.
When using higher concentrations of DIG-deoxyuridinetriphosphate (dUTP) what is supplied with the PCR DIG Labeling Mix, the yield of the PCR product may be reduced, but however, the label intensity and the molecular weight of the PCR product is increased.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
The PCR DIG Labeling Mix is specifically designed for the sensitive detection of polymerase chain reaction (PCR) products and the sensitive analysis of PCR reactions. The PCR DIG Labeling Mix can also be used for the synthesis of hybridization probes. However, for the production of highly sensitive probes, for example, necessary when detecting single-copy genes on genomic blots, we recommend a PCR nucleotide mix with an increased concentration of DIG-dUTP (e.g., PCR DIG Probe Synthesis Kit).
PCR DIG Labeling Mix has been used in the preparation of digoxigenin-labeled riboprobes during the in vitro transcription step.
PCR DIG Labeling Mix has been used in the preparation of digoxigenin-labeled riboprobes during the in vitro transcription step.
Quality
The PCR DIG Labeling Mix is function tested in PCR. Amplification products are assayed by dot blot and in hybridization experiments. DNases and RNases are not detectable according to the current Quality Control procedures.
Physical form
Solution, 10x concentrated: PCR DIG labeling mix is a mixture of the sodium salts of dATP, dCTP, dGTP, dTTP and digoxigenin-11-dUTP, lithium salt. The solution contains 2 mM dATP, dCTP, dGTP each, 1.9 mM dTTP, 0.1 mM digoxigenin-11-dUTP (DIG-11-dUTP) in 2x 250 μl water; pH 7.0.
Other Notes
For life science research only. Not for use in diagnostic procedures.
also commonly purchased with this product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Yukihiro Kabeya et al.
Plant physiology, 161(4), 2102-2112 (2013-03-01)
Chloroplasts arose from a cyanobacterial endosymbiont and multiply by division. In algal cells, chloroplast division is regulated by the cell cycle so as to occur only once, in the S phase. Chloroplasts possess multiple copies of their own genome that
Dawei Zhang et al.
Oncology letters, 5(5), 1694-1698 (2013-06-14)
In view of the previously demonstrated clinical role of the anti-latent membrane protein 1 (LMP1) immunoconjugate HLEAFab-MMC in the treatment of advanced nasopharyngeal carcinoma (NPC), reliable detection of LMP1 expression is of key importance. The aim of this study was
Ikuo K Suzuki et al.
Cell, 173(6), 1370-1384 (2018-06-02)
The cerebral cortex underwent rapid expansion and increased complexity during recent hominid evolution. Gene duplications constitute a major evolutionary force, but their impact on human brain development remains unclear. Using tailored RNA sequencing (RNA-seq), we profiled the spatial and temporal expression
Krishna S Ghanta et al.
eLife, 10 (2021-10-20)
Nuclease-directed genome editing is a powerful tool for investigating physiology and has great promise as a therapeutic approach to correct mutations that cause disease. In its most precise form, genome editing can use cellular homology-directed repair (HDR) pathways to insert
Radka Symonová et al.
BMC evolutionary biology, 13, 42-42 (2013-02-16)
Sympatric species pairs are particularly common in freshwater fishes associated with postglacial lakes in northern temperate environments. The nature of divergences between co-occurring sympatric species, factors contributing to reproductive isolation and modes of genome evolution is a much debated topic
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service