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Key Documents

MAB3632

Sigma-Aldrich

Anti-PPAR gamma 1 and 2 Antibody, phospho-specific (Ser112)

ascites fluid, Chemicon®

Synonym(s):

PPARgamma1 and 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

phosphorylation (pSer112)

Gene Information

human ... PPARG(5468)

Specificity

Recognizes phosphorylated Peroxisome Proliferator Activated Receptor (PPAR) gamma1 and gamma2.

Immunogen

Epitope: phospho-specific (Ser112)
Phosphorylated (serine 112) synthetic peptide from amino acids 107-121 of human PPAR gamma2 (identical to PPAR gamma1). No homology with PPAR alpha or PPAR beta.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Use Anti-PPAR gamma 1 & 2 Antibody, phospho-specific (Ser112) (mouse monoclonal antibody) validated in ELISA, WB, ICC to detect PPAR gamma 1 & 2 also known as Peroxisome Proliferator Activated Receptor γ 1 & 2.
Western blot: 1:500-1:5,000 on endogenous and recombinant protein.

Immunocytochemistry: 1:500-1:5,000

ELISA: 1:500-1:5,000

Optimal working dilutions must be determined by the end user.

Physical form

Ascites. Liquid. Contains no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Tobias B Huber et al.
Molecular and cellular biology, 23(14), 4917-4928 (2003-07-02)
Mutations of NPHS1 or NPHS2, the genes encoding nephrin and podocin, as well as the targeted disruption of CD2-associated protein (CD2AP), lead to heavy proteinuria, suggesting that all three proteins are essential for the integrity of glomerular podocytes, the visceral
Dalila El Ouarrat et al.
Cell metabolism, 31(1), 162-173 (2019-11-12)
Insulin resistance is a major factor in obesity-linked type 2 diabetes. PPARγ is a master regulator of adipogenesis, and small molecule agonists, termed thiazolidinediones, are potent therapeutic insulin sensitizers. Here, we studied the role of transcriptional co-activator with PDZ-binding motif
Bo-Mi Kim et al.
International journal of molecular medicine, 43(1), 489-500 (2018-10-27)
Muscat Bailey A grape stalk is an organic waste produced in marked amounts during the vinification of grapes. Previous studies have indicated that grape stalk is rich in bioactive phenolic compounds, and exhibits antioxidant and UV‑protective activities. However, its effects
Larry A Denner et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(47), 16725-35a-16725-35a (2012-11-24)
We previously reported that the peroxisome proliferator-activated receptor γ (PPARγ) agonist rosiglitazone (RSG) improved hippocampus-dependent cognition in the Alzheimer's disease (AD) mouse model, Tg2576. RSG had no effect on wild-type littermate cognitive performance. Since extracellular signal-regulated protein kinase mitogen-activated protein
Keisuke Yanagida et al.
JCI insight, 3(24) (2018-12-21)
White adipose tissue (WAT) can dynamically expand and remodel through adipocyte hypertrophy and hyperplasia. The relative contribution of these 2 mechanisms to WAT expansion is a critical determinant of WAT function and dysfunction in obesity. However, little is known about

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