1.50453

SeQuant^® ZIC^®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column, PEEK-lined stainless steel

• Synonym(s): Zwitterionic Hydrophilic Interaction Liquid Chromatography HPLC Column
• UNSPSC Code: 41115709

Properties

• product name: SeQuant^® ZIC-HILIC^™ (5µm,200Å) PEEK 100 x 4.6 mm, suitable for HPLC
• material: PEEK-lined stainless steel column
• Quality Level: 100
• Agency: suitable for USP L114
• product line: SeQuant^®
• feature: endcapped: no
• parameter: 500 bar pressure; 70 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 10 cm × 4.6 mm
• matrix: fully porous particle
• matrix active group: ZIC-HILIC bonding phase
• particle size: 5 μm
• pore size: 200 Å pore size
• operating pH: 2-7.5
• separation technique: hydrophilic interaction (HILIC)
• storage temp.: no temp limit

Description

General description

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Application

• Development of a HILIC method for the determination of 5-fluorouracil from nano drug delivery systems and rat skin extracts.: This study focuses on developing a HILIC method using SeQuant^® ZIC-HILIC^™ for the determination of 5-fluorouracil. The method was applied to analyze samples from nano drug delivery systems and rat skin extracts, highlighting its utility in pharmaceutical and biomedical research (Amasya et al., 2018).

Analysis Note

Theoretical Plates (N/m) (Cytosine): ≥ 60000
Symmetry (Cytosine): 0.95 - 1.40

Legal Information

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany