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04-1555

Sigma-Aldrich

Anti-Mdmx Antibody, clone 8C6

clone 8C6, from mouse

Synonym(s):

Double minute 4 protein, MDM4-related protein 1, Mdm2-like p53-binding protein, Mdm4 p53 binding protein homolog (mouse), Mdm4, transformed 3T3 cell double minute 4, p53 binding protein, Mdm4, transformed 3T3 cell double minute 4, p53 binding protein (mo

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8C6, monoclonal

species reactivity

human

technique(s)

ChIP: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MDM4(4194)

Related Categories

General description

Mdmx is a Mdm2-related protein that like Mdm2 has been shown to bind to and inhibit p53, although not as well as Mdm2. This interaction with p53 occurs via a binding domain located in the N-terminal region of the protein. Mdmx has been shown to counteract Mdm2-dependent p53 degradation and to stabilize p53 in its inactive form. Although Mdmx has significant structural similarity to Mdm2 E3 ubiquitin ligase, most studies have not been able to ascribe E3 ligase function to Mdmx.

Specificity

This antibody recognizes Mdmx.

Immunogen

Epitope: Unknown
His-tagged recombinant protein corresponding to human Mdmx.

Application

Anti-Mdmx Antibody, clone 8C6 is a Mouse Monoclonal Antibody for detection of Mdmx also known as MDM4-related protein 1, Mdm2-like p53-binding protein & has been validated in WB, IP, ICC, IHC, FC.
Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory for this application. (Gilkes, D., et al. (2008). Molecular and Cellular Biology. 28(6):1999-2010.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratoryfor this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)

Flow Cytometry Analysis: A previous lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in MCF-7 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Mdmx on 10 µg of MCF-7 cell lysate.

Target description

~ 80 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
MCF-7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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