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Key Documents

G005716

HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
mobile phase: [A] 5 mM ammonium phosphate monobasic, pH 2.0 with phosphoric acid: acetonitrile (98:2); [B] acetonitrile;
gradient: 0 to 68% B in 3 min; held at 68% B for 2 min
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: UV, 210 nm
injection: 1 μL
sample: 100 μg/mL in 95:5, water: methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Analysis Note

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of various over the counter drugs.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Maleic acid
    Acetaminophen
    Caffeine
    Naproxen
    Ibuprofen

analytical column

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standard

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