58238
SUPELCOSIL™ LC-8 (5 µm) HPLC Columns
L × I.D. 5 cm × 4.6 mm, HPLC Column
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UNSPSC Code:
41115700
eCl@ss:
32110501
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Product Name
SUPELCOSIL™ LC-8 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
Agency
suitable for USP L7
Quality Level
feature
endcapped
manufacturer/tradename
SUPELCOSIL™
extent of labeling
6.0% carbon loading
parameter
≤70 °C temp. range
400 bar pressure (5801 psi)
technique(s)
HPLC: suitable
L × I.D.
5 cm × 4.6 mm
surface area
170 m2/g
surface coverage
surface coverage 3.2 μmol/m2
matrix
silica gel, spherical particle platform
matrix active group
C8 (octyl) phase
particle size
5 μm
pore size
120 Å
pH range
2-7.5
application(s)
food and beverages
separation technique
reversed phase
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General description
A phase less hydrophobic than C18. Provides less retention of both polar and non-polar compounds than C18. Use a mobile phase containing 5% less organic modifier for the C8 column than C18. Polar compounds are, relatively, more strongly retained on C8 than C18 columns.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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F Belal et al.
Journal of pharmaceutical and biomedical analysis, 24(3), 335-342 (2001-02-24)
A simple, rapid and sensitive HPLC method has been developed for the simultaneous determination of ramipril and hydrochlorothiazide in their dosage forms. Acetonitrile: sodium perchlorate solution (0.1 M) adjusted to pH 2.5+/-0.2 with phosphoric acid (46:54 v/v), was used as
Thomas Borch et al.
Journal of chromatography. A, 1022(1-2), 83-94 (2004-02-03)
Explosives such as 2,4,6-trinitrotoluene (TNT), octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine (HMX), and hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) are widely distributed environmental contaminants. Complete chromatographic separation is necessary in order to accurately determine and quantify explosives and their degradation products in environmental samples and in (bio)transformation studies. The
S B Ruddy et al.
Journal of chromatography. B, Biomedical applications, 657(1), 83-92 (1994-07-01)
The isocratic, reversed-phase, high-performance liquid chromatographic (HPLC) method presented provides a simple and rapid analytical technique for the simultaneous determination of low-molecular-mass oligomers of polyethylene glycol (PEG) in aqueous polymer samples containing polar contaminants of biologic origin. In the present
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
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