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Key Documents

T3134

Sigma-Aldrich

N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride

≥95%, powder

Synonym(s):

TMPPD, Wurster’s reagent

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About This Item

Linear Formula:
C6H4[N(CH3)2]2·2HCl
CAS Number:
Molecular Weight:
237.17
Beilstein:
7442241
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

Quality Level

Assay

≥95%

form

powder

color

off-white to gray-brown

mp

222-224 °C (lit.)

solubility

H2O: 500 mg/10 mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].Cl[H].CN(C)c1ccc(cc1)N(C)C

InChI

1S/C10H16N2.2ClH/c1-11(2)9-5-7-10(8-6-9)12(3)4;;/h5-8H,1-4H3;2*1H

InChI key

FBHKTSXMTASXFJ-UHFFFAOYSA-N

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General description

N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD) is a redox mediator used in the oxidase test for detecting bacterial cytochrome c oxidases. TMPD is commonly used to perform the oxidase test for the colorimetric identification of pathogenic and non-pathogenic bacteria.

Application

N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride has been used to determine the oxidase activity displayed by various microorganisms.

Caution

May darken in storage.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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