SAB4200489

Anti-Occludin (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

• Synonym(s): Anti-OCLN
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~65 kDa
• species reactivity: human, dog
• concentration: ~1.0 mg/mL
• technique(s): immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin-embedded human colon.; indirect immunofluorescence: 1-2 μg/mL using MDCK cells; western blot: 1.5-3.0 μg/mL using extracts of Caco-2 cells
• UniProt accession no.: Q16625
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... OCLN(100506658)

Description

General description

Occludin (OCLN) is an integral membrane protein, encoded by the gene mapped to human chromosome 5q13.2. OCLN has a molecular weight of about 65kDa. It possesses four transmembrane domains including a long COOH-terminal cytoplasmic domain, a short NH2-terminal cytoplasmic domain, two extracellular loops and one intracellular turn. It is specifically localized at tight junctions (TJ).

Immunogen

a synthetic peptide corresponding to a sequence at the C-terminal region of human occludin, conjugated to KLH. The corresponding sequence is highly conserved in rat (89% sequence identity) and in mouse (84% sequence identity) occludin.

Application

Anti-Occludin (C-terminal) antibody produced in rabbit has been used in immunohistochemistry.

Biochem/physiol Actions

Occludin (OCLN), along with tight junction (TJ)-associated peripheral membrane proteins plays a vital role in the formation and regulation of TJ. The encoded protein facilitates the translocation of p85a (regulatory subunit of phosphoinositide 3-kinase) to TJs to regulate actin organization after oxidative stress. OCLN is also implicated in cell migration. Mutation in the gene leads to the development of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable