Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
P3644
L-α-Phosphatidylcholine
from soybean, Type IV-S, ≥30% (enzymatic)
Synonym(s):
1,2-Diacyl-sn-glycero-3-phosphocholine, 3-sn-Phosphatidylcholine, L-α-Lecithin, Azolectin, PC
About This Item
Recommended Products
biological source
soybean
Quality Level
type
Type IV-S
form
solid
concentration
≥30% (enzymatic)
solubility
chloroform: soluble 100 mg/mL, clear to slightly hazy, yellow to orange
functional group
phospholipid
lipid type
phosphoglycerides
shipped in
ambient
storage temp.
−20°C
SMILES string
[P](=O)([O-])(OC[C@H](OC(=O)CCCCCCC\C=C/C\C=C/CCCCC)COC(=O)CCCCCCCCCCCCCCC)OCC[N+](C)(C)C
InChI
1S/C42H80NO8P/c1-6-8-10-12-14-16-18-20-21-23-25-27-29-31-33-35-42(45)51-40(39-50-52(46,47)49-37-36-43(3,4)5)38-48-41(44)34-32-30-28-26-24-22-19-17-15-13-11-9-7-2/h14,16,20-21,40H,6-13,15,17-19,22-39H2,1-5H3/b16-14-,21-20-/t40-/m1/s1
InChI key
JLPULHDHAOZNQI-ZTIMHPMXSA-N
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Application
- as a component of human erythroid massive amplification culture (HEMAdef) medium for clinical expansion of human erythroblasts[1]
- in the chloride efflux assay to examine the chloride permeability of lipid vesicles conferred by soluble CLIC1[2]
- in an assay to measure solubilized wax synthase activity[3]
- as a component of buffer A used for the resuspension of the cholate-solubilized catalytic unit of adenylate cyclase[4]
- as a cryoprotectant for freezing ruminant sperm[5]
- as a substrate for the estimation of phospholipase activity[6]
- to make liposomes to assess their efficiency of recruitment of ARF (ADP ribosylation factor) and AP-1 (adaptor protein)[7]
- in the preparation of the isolated catalytic unit of soluble adenylate cyclase[8]
Biochem/physiol Actions
Preparation Note
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Customers Also Viewed
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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It is said that it has also been used in cell culture, but is Chloroform or Ether harmless in cell culture? Also, is it completely insoluble in other organic solvents such as Chloroform, Ether, and DMSO?
1 answer-
The product's solubility is tested in chloroform at a concentration of 100 mg/mL. Purified egg L-α-phosphatidylcholine is soluble (100 mg/mL) at room temperature in chloroform, ethanol, and hexane containing 3% ethanol. These solubility characteristics should generally apply to other purified natural product phosphatidylcholine products, such as those from soybean and brain, that contain unsaturated fatty acids. The product's solubility in dimethyl sulfoxide has not been determined. It is advised to check specific cells or cell lines to determine their sensitivity to solvents such as dimethyl sulfoxide, chloroform, and ether.
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What kind of product is it, a powder?
1 answer-
This product is listed as a solid but can range in appearance from waxy to powdery. The consistency is not a tested product specification and is simply reported as a solid. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/P3644-100G0000285416.pdfHelpful?
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