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P3134

Sigma-Aldrich

Phosphodiesterase I from Crotalus adamanteus venom

Type VI, crude dried venom

Synonym(s):

5′-Exonuclease, Oligonucleate 5′-nucleotidohydrolase

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type VI

Quality Level

form

crude dried venom

storage temp.

−20°C

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General description

Research area: Cell signaling. PhosphodiesteraseI (PDEI) exists in three isoforms PDE1A, PDE1B, and PDE1C(2) are localized to the central nervous system, cardiovascular, and other organs.

Application

Phosphodiesterase I from Crotalus adamanteus venom has been used:
  • for cleaving the 3′-5′ internucleotide bonds in the linear chains of the RNA oligomers
  • as a model for the hydrolysis of prodrugs in phosphodiesterase assay
  • as a component in the nucleotide cleavage buffer

Biochem/physiol Actions

Phosphodiesterase I breaks phosphodiester bonds and catalyzes the hydrolysis of various nucleotide polyphosphates. Phosphodiesterase I is released from eucaryotic plasma membranes by phosphatidylinositol-specific phospholipase C. PhosphodiesteraseI (PDEI) can hydrolyze 3′,5′-cyclic adenosine monophosphate (cAMP) and3′,5′-cyclic guanosine monophosphate (cGMP). It binds to Ca2+/calmodulin(CaM) that lead to the stimulation of its catalytic activity.PDEIacts as a biotherapeutic for the treatment of cancer, heart diseases,pulmonary, metabolic, neurocognitive, renal, endocrine abnormalities, andneurological disorders.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ester prodrugs of acyclic nucleoside thiophosphonates compared to phosphonates: synthesis, antiviral activity and decomposition study
Roux L, et al.
European Journal of Medicinal Chemistry, 63, 869-881 (2013)
Anatoly I Dragan et al.
Nucleic acids research, 32(17), 5192-5197 (2004-10-02)
The interaction of proteins with DNA results, in some cases, in DNA bending, and this might have functional importance. However, when the protein-induced bending of DNA is small, its measurement presents a problem. It is shown that the fluorescence resonance
Ozone-Induced DNA Damage: A Pandora?s Box of Oxidatively Modified DNA Bases
Richard W J, et al.
Chemical Research in Toxicology (2021)
Ralph Theo Schermuly et al.
Circulation, 115(17), 2331-2339 (2007-04-18)
Pulmonary arterial hypertension (PAH) is a life-threatening disease, characterized by vascular smooth muscle cell hyperproliferation. The calcium/calmodulin-dependent phosphodiesterase 1 (PDE1) may play a major role in vascular smooth muscle cell proliferation. We investigated the expression of PDE1 in explanted lungs
Formation of RNA oligonucleotides over the mineral surface preliminary irradiated with UV light
Otroshchenko VA and Vasilyeva NV
Reaction Kinetics and Catalysis Letters, 97(1), 151-156 (2009)

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