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Key Documents

P3111

Sigma-Aldrich

Protease from Bacillus sp.

liquid, ≥16 U/g

Synonym(s):

Savinase® 16.0L

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About This Item

EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Bacillus sp.

Quality Level

form

liquid

specific activity

≥16 U/g

mol wt

20-30 kDa

storage temp.

2-8°C

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Application

Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Product P3111 is secreted from Bacillus sp.. It is a serine-type protease characterized by excellent performance at elevated temperature and pH. Product P3111 is extremely stable under harsh storage conditions, such as in the presence of sodium hypochlorite. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics.

Biochem/physiol Actions

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Proteases, secreted from Bacillus sp., typically have molecular weights ranging from 20,000 to 30,000. They are typically stabilized by Ca2+ and have high isoelectric points. It is highly soluble in detergents at a wide range of pH and temperatures.

Legal Information

A product of Novozyme Corp.
Savinase is a registered trademark of Novozymes Corp.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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