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Key Documents

N3641

Sigma-Aldrich

4-Nitrophenyl α-L-arabinofuranoside

chromogenic, ≥98% (TLC), powder

Synonym(s):

4-nitrophenyl-ARA

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About This Item

Empirical Formula (Hill Notation):
C11H13NO7
CAS Number:
Molecular Weight:
271.22
Beilstein:
1688357
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

4-Nitrophenyl α-L-arabinofuranoside, ≥98% (TLC)

Quality Level

Assay

≥98% (TLC)

form

powder

solubility

methanol: 50 mg/mL

storage temp.

−20°C

SMILES string

OC[C@@H]1O[C@@H](Oc2ccc(cc2)[N+]([O-])=O)[C@H](O)[C@H]1O

InChI

1S/C11H13NO7/c13-5-8-9(14)10(15)11(19-8)18-7-3-1-6(2-4-7)12(16)17/h1-4,8-11,13-15H,5H2/t8-,9-,10+,11+/m0/s1

InChI key

DUYYBTBDYZXISX-UKKRHICBSA-N

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Application

4-Nitrophenyl α-L-arabinofuranoside has been used:
  • as a substrate for α-arabinofuranosidase in an enzymatic assay to determine glycosidase activities in Oenococcus oeni strains
  • to determine the enzymatic activity of α-arabinofuranosidase
  • as a p-nitrophenol linked substrate to determine its activity in Aspergillus niger NW249 culture filtrate

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Joost van den Brink et al.
Biotechnology journal, 9(10), 1329-1338 (2014-08-15)
Plant-degrading enzymes can be produced by fungi on abundantly available low-cost plant biomass. However, enzymes sets after growth on complex substrates need to be better understood, especially with emphasis on differences between fungal species and the influence of inhibitory compounds
B C Saha et al.
Applied and environmental microbiology, 64(1), 216-220 (1998-01-22)
A color-variant strain of Aureobasidium pullulans (NRRL Y-12974) produced alpha-L-arabinofuranosidase (alpha-L-AFase) when grown in liquid culture on oat spelt xylan. An extracellular alpha-L-AFase was purified 215-fold to homogeneity from the culture supernatant by ammonium sulfate treatment, DEAE Bio-Gel A agarose
José Manuel Inácio et al.
Journal of bacteriology, 190(12), 4272-4280 (2008-04-15)
The extracellular depolymerization of arabinopolysaccharides by microorganisms is accomplished by arabinanases, xylanases, and galactanases. Here, we characterize a novel endo-alpha-1,5-l-arabinanase (EC 3.2.1.99) from Bacillus subtilis, encoded by the yxiA gene (herein renamed abn2) that contributes to arabinan degradation. Functional studies
Liudmila V Kozlova et al.
Planta, 241(5), 1159-1172 (2015-01-23)
Specific α- l -arabinofuranosidases are involved in the realisation of elongation growth process in cells with type II cell walls. Elongation growth in a plant cell is largely based on modification of the cell wall. In type II cell walls
M J Renner et al.
Applied and environmental microbiology, 64(1), 43-52 (1998-01-22)
An alpha-L-arabinofuranosidase (alpha-L-arabinofuranoside arabinofuranohydrolase [EC 3.2.1.55]; referred to below as ArfI) from Cytophaga xylanolytica XM3 was purified 85-fold by anion-exchange and hydrophobic interaction column chromatography. The native enzyme had a pI of 6.1 and an apparent molecular mass of 160

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