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MAK112

Sigma-Aldrich

Arginase Activity Assay Kit

sufficient for 200 colorimetric tests

Synonym(s):

Arginase Test Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 200 colorimetric tests

detection method

colorimetric

relevant disease(s)

cancer; immunological diseases; gastrointestinal diseases

storage temp.

−20°C

Gene Information

human ... ARG1(383) , ARG2(384)
mouse ... ARG1(11846) , ARG2(11847)
rat ... ARG2(29215) , Arg1(29221)

General description

Arginase is a manganese-containing enzyme that catalyzes the conversion of arginine to urea and ornithine. Two isoforms of arginase are present in most mammals which differ in their tissue distribution and subcellular localization. Arginase I is a cytoplasmic protein predominantly expressed in the liver where it catalyzes the fifth and final step of the Urea Cycle. Arginase II is a mitochondrial protein with broader tissue distribution whose function may include nitric oxide and polyamine metabolism. Decreased activity or expression of Arginase I results in the autosomal recessive disorder hyperarginemia. Increased serum Arginase activity is implicated in hepatic injury and in certain pathological conditions such as cancer. Arginase also participates in multiple inflammatory reactions in the immune system, such as macrophage-mediated cytotoxicity.

Application

Arginase Activity Assay Kit has been used to assess the activity of arginase.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of arginase activity in enzyme preparations, serum, plasma, tissue culture, and similar samples. Suitable for studying the effects of drugs on arginase activity.

Principle

In this assay, arginase catalyzes the conversion of arginine to urea and ornithine. The urea produced specifically reacts with the substrate to generate a colored product, proportional to the arginase activity present.

Pictograms

Health hazardCorrosion
GHS08,GHS05

Signal Word

Danger

Hazard Statements

H290,H314,H360FD,H412

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1A

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Arginase activity, urea, and hydroxyproline concentration are reduced in keratoconus keratocytes.
Stachon T, et al.
Graefe'S Archive For Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie, 255(1), 91-97 (2017)
Razieh Tavakoli Oliaee et al.
Microbial pathogenesis, 126, 368-378 (2018-11-07)
Detection of the mechanism of host/parasite interactions in unresponsive forms of anthroponotic cutaneous leishmaniasis (ACL) caused by Leishmania tropica is helpful for immunotherapy and vaccine development. In the present study, the gene expression of toll-like receptors (TLRs), TNF-α, iNOS and
Clinical phenotype, biochemical profile, and treatment in 19 patients with arginase 1 deficiency.
Martina H.
Journal of inherited metabolic disease, 39(3), 331?340-331?340 (2016)
Yuxin Shu et al.
Immunology, 160(4), 345-356 (2020-04-21)
Macrophages are particularly abundant and play an important role throughout the tumor progression process, namely, tumor-associated macrophages (TAM) in the tumor microenvironment. TAM can be polarized to disparate functional phenotypes, the M1 and M2 macrophages. M1-like type macrophages are defined
Andrew F Cumpstey et al.
Nitric oxide : biology and chemistry, 94, 27-35 (2019-10-12)
Native highlanders (e.g. Sherpa) demonstrate remarkable hypoxic tolerance, possibly secondary to higher levels of circulating nitric oxide (NO) and increased microcirculatory blood flow. As part of the Xtreme Alps study (a randomised placebo-controlled trial of dietary nitrate supplementation under field
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