L3147
LB Broth with agar (Miller)
Highly-referenced nutrient-rich microbial growth powder medium with Agar, suitable for regular E.coli culture.
Synonym(s):
Luria Agar, Luria Bertani agar, Miller’s LB agar, lysogeny broth agar
About This Item
Recommended Products
grade
for molecular biology
Quality Level
sterility
non-sterile
form
powder
composition
Agar, 15 g/L
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
technique(s)
microbiological culture: suitable
pH
6.8-7.2(4% solution)
application(s)
food and beverages
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
Application
Features and Benefits
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
Preparation Note
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into sterile petri dishes.
To prepare the medium of Luria and Burrows: Add 1 g glucose to medium and proceed with preparation instructions above.
To prepare the medium of Luria, Adams and Ting (also known as LC agar): Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
Reconstitution
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Articles
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Protocols
General protocols for growth of competent cells in microbial medium.
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