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HYSC020

Sigma-Aldrich

HyStem®-C Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution

Synonym(s):

Cell Culture Scaffold, HyStem-C Scaffold Kit

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96 WELLS
JP¥88,000

JP¥88,000


Estimated to ship on12 March 2025

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96 WELLS
JP¥88,000

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

JP¥88,000


Estimated to ship on12 March 2025

New, lower price on this item!

Request a Bulk Order

Quality Level

storage temp.

−20°C

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1 of 4

This Item
RAB1255RAB1532RAB1068
Gene Information

human ... LY96(23643)

Gene Information

human ... BST1(683)

Gene Information

human ... LILRB1(10859)

Gene Information

human ... KNG1(3827)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1.6 ng/ml

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.06 ng/mL
standard curve range: 0.051-12.50 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 22 pg/mL
standard curve range: 20.48-5000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 24 ng/ml

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum sample(s)

input

sample type cell culture supernatant(s)
sample type plasma
sample type serum

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

sample type plasma
sample type serum sample(s)
sample type cell culture supernatant(s)

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

The HyStem® -C Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture. It is recommended for cultures, which require a minimal number of cell attachment sites or the addition of other extracellular matrix (ECM) proteins.

Application

HyStem® -C cell culture scaffold kit has been used for neural stem cell culture.[1]

Features and Benefits

Customizable, well characterized and consistent, Hystem-C hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-C offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-C offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-C contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-C hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.
Hystem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-C, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-C has been successfully used to expand human embryonic stem cells, human mesenchymal stem cells, neural progenitor cells and hepatic progenitor cells.

Legal Information

Hystem is a registered trademark of BIOTIME, INC

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids


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    Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
    Basuodan R, et al.
    Journal of Anatomy (2018)
    Reem Basuodan et al.
    Journal of anatomy, 233(2), 155-166 (2018-05-11)
    Human neural stem cells (hNSC) derived from induced pluripotent stem cells can be differentiated into neurons that could be used for transplantation to repair brain injury. In this study we dispersed such hNSC in a three-dimensional artificial extracellular matrix (aECM)
    Human neural stem cells dispersed in artificial ECM form cerebral organoids when grafted in vivo
    Basuodan R, et al.
    Journal of Anatomy (2018)
    Douglas O Sobel et al.
    Islets, 12(4), 71-86 (2020-06-23)
    Immortalized beta cells are an abundant source of insulin-producing cells. Although MIN-6 cells have similar characteristics as normal islets in vitro, the in vivo use of MIN-6 cells has not been fully described. This study characterizes in vivo mouse models

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