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Key Documents

C0918

Supelco

Fluorimeter cuvettes

Polystyrene, without stopper

Synonym(s):

cuvette, plastic cuvette, plastic cuvettes

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About This Item

UNSPSC Code:
12000000
NACRES:
SB.32

Quality Level

100

parameter

340-750 nm spectral range

technique(s)

photometry: suitable

L × W × H

12.7 mm × 12.7 mm × 44.7 mm

pathlength

10 mm

volume

≥3 mL

detection method

fluorometric

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General description

See transmission curve labelled D
These cuvettes have four optically clear sides.

Note: polystyrene and polymethacrylate cuvettes are incompatible with some organic solvents.

Application

Fluorimeter cuvette may be used :
  • to perform a fluorimetric assay of the hepatic microsomal O-dealkylation reaction, using ethoxyresorufin (7-ethoxyphenoxazone) as a model substrate for the assay.
  • in the cationic liposome-mediated gene transfer method, done in order to deduce the role of helper lipids.
  • in the fluorescence experiment modified in the Silber-Porter method, for estimation of corticosterone in rat plasma.
  • in fluorimeteric assay for membrane potential to determine membrane depolarization in study done to elucidate the role of membrane potential in the mechanism of action of daptomycin.

Compatibility

Not compatible with some organic solvents.

Certificates of Analysis (COA)

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The estimation of small amounts of corticosterone in rat plasma.
N ZENKER et al.
The Journal of biological chemistry, 231(2), 695-701 (1958-04-01)
Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene.
M D Burke et al.
Drug metabolism and disposition: the biological fate of chemicals, 2(6), 583-588 (1974-11-01)
S W Hui et al.
Biophysical journal, 71(2), 590-599 (1996-08-01)
In the procedure for cationic liposome-mediated transfection, the cationic lipid is usually mixed with a "helper lipid" to increase its transfection potency. The importance of helper lipids, including dioleoylphosphatidylcholine (DOPC) and phosphatidylethanolamine (dioleoyl PE), DO was examined. Freeze-fracture electron microscopy
Jared A Silverman et al.
Antimicrobial agents and chemotherapy, 47(8), 2538-2544 (2003-07-25)
The objective of this study was to further elucidate the role of membrane potential in the mechanism of action of daptomycin, a novel lipopeptide antibiotic. Membrane depolarization was measured by both fluorimetric and flow cytometric assays. Adding daptomycin (5 micro

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