B6008

Ponceau BS

Dye content ~60 %, Powder

• Synonym(s): Acid Red 66, Biebrich scarlet WS, Biebrich scarlet sodium salt
• Empirical Formula (Hill Notation): C₂₂H₁₄N₄Na₂O₇S₂
• CAS Number: 4196-99-0
• Molecular Weight: 556.48
• Colour Index Number: 26905
• Beilstein: 3883900
• EC Number: 224-084-5
• MDL number: MFCD00003891
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891888
• NACRES: NA.47

Properties

• product name: Ponceau BS, Dye content ≥55 %
• form: powder
• composition: Dye content, ≥55%
• technique(s): microbe id | staining: suitable
• color: red to brown
• solubility: ethanol: 1 mg/mL; H₂O: >30 mg/mL
• εmax: ≥19000 at 510 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].Oc1ccc2ccccc2c1\N=N\c3ccc(cc3S([O-])(=O)=O)\N=N\c4ccc(cc4)S([O-])(=O)=O
• InChI: 1S/C22H16N4O7S2.2Na/c27-20-12-5-14-3-1-2-4-18(14)22(20)26-25-19-11-8-16(13-21(19)35(31,32)33)24-23-15-6-9-17(10-7-15)34(28,29)30;;/h1-13,27H,(H,28,29,30)(H,31,32,33);;/q;2*+1/p-2/b24-23+,26-25+;;
• InChI key: VVAVKBBTPWYADW-RVTJCSDESA-L

Description

Application

Ponceau BS has been used in Masson′s trichrome stain for the staining of collagen. It has also been used for eosinophil staining.

Biochem/physiol Actions

Ponceau BS is used in Masson′s trichrome staining procedure, along with acid fuchsin, for the labeling of collagen and muscle red.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves