A5268

Alcian Blue 8GX

powder

• Synonym(s): Alcian Blue, Ingrain Blue 1
• Empirical Formula (Hill Notation): C₅₆H₆₈Cl₄CuN₁₆S₄
• CAS Number: 33864-99-2
• Molecular Weight: 1298.86
• Colour Index Number: 74240
• EC Number: 251-705-7
• MDL number: MFCD00010720
• UNSPSC Code: 12171500
• PubChem Substance ID: 24890962
• NACRES: NA.47

Properties

• form: powder
• composition: Dye content, 45-65%
• technique(s): microbe id | staining: suitable
• color: blue
• solubility: H₂O: 1 mg/mL
• εmax: 425 at 615-630 nm in water
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Cl-].[Cl-].[Cl-].[Cl-].CN(C)C(\SCc1ccc2c3nc(nc4n5[Cu]n6c(nc7nc(nc5c8cc(CS\C(N(C)C)=[N+](\C)C)ccc48)c9ccc(CS\C(N(C)C)=[N+](\C)C)cc79)c%10ccc(CS\C(N(C)C)=[N+](\C)C)cc%10c6n3)c2c1)=[N+](/C)C
• InChI: 1S/C56H68N16S4.4ClH.Cu/c1-65(2)53(66(3)4)73-29-33-17-21-37-41(25-33)49-57-45(37)62-50-43-27-35(31-75-55(69(9)10)70(11)12)19-23-39(43)47(59-50)64-52-44-28-36(32-76-56(71(13)14)72(15)16)20-24-40(44)48(60-52)63-51-42-26-34(18-22-38(42)46(58-51)61-49)30-74-54(67(5)6)68(7)8;;;;;/h17-28H,29-32H2,1-16H3;4*1H;/q+2;;;;;+2/p-4
• InChI key: CKLBXIYTBHXJEH-UHFFFAOYSA-J

Description

General description

Alcian Blue 8GX, also known as Ingrain Blue 1, is a basic copper phthalocyanine dye whose copper content imparts a blue color. It is tetravalent, cationic, and water-soluble in nature. It forms salt linkages with the acid groups of acid mucopolysaccharides.

Application

• Alcian Blue 8GX has been used for the staining and precipitation of glycosaminoglycans and as a heteroglycan stain for neutral, sulfated, and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.
• It has been employed in the in vivo visualization of intravascular Bonghan ducts and corpuscles in murine models.
• It has been used to develop a novel histochemical staining method to assess multiple respiratory parameters in various species.

Features and Benefits

Desired staining patterns can be obtained by manipulating pH and salt concentrations.

Principle

The Alcian blue staining is easily combined with the Periodic acid-Schiff (PAS) reaction. In the tissue, acid mucosubstances are stained selectively and nuclear fast red is commonly used as counterstain. In combination with the PAS reaction, non-substituted polysaccharides, neutral mucopolysaccharides, muco- and glycoproteins, and glycol- and phospholipids can be visualized in addition. An Acetic acid-Alcian blue solution is used for the staining, whereby carboxylated and sulfatic proteoglycans are selectively stained when a pH of 2.5 is applied. The differentiation between carboxyl and sulfate groups is only possible at pH 1.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves